EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM Structures Reveal Relocalization of MetAP in the Presence of Other Protein Biogenesis Factors at the Ribosomal Tunnel Exit.
ジャーナル・号・ページ	J Mol Biol, Vol. 431, Issue 7, Page 1426-1439, Year 2019
掲載日	2019年3月29日
著者	Sayan Bhakta / Shirin Akbar / Jayati Sengupta /
PubMed 要旨	During protein biosynthesis in bacteria, one of the earliest events that a nascent polypeptide chain goes through is the co-translational enzymatic processing. The event includes two enzymatic ...During protein biosynthesis in bacteria, one of the earliest events that a nascent polypeptide chain goes through is the co-translational enzymatic processing. The event includes two enzymatic pathways: deformylation of the N-terminal methionine by the enzyme peptide deformylase (PDF), followed by methionine excision catalyzed by methionine aminopeptidase (MetAP). During the enzymatic processing, the emerging nascent protein likely remains shielded by the ribosome-associated chaperone trigger factor. The ribosome tunnel exit serves as a stage for recruiting proteins involved in maturation processes of the nascent chain. Co-translational processing of nascent chains is a critical step for subsequent folding and functioning of mature proteins. Here, we present cryo-electron microscopy structures of Escherichia coli (E. coli) ribosome in complex with the nascent chain processing proteins. The structures reveal overlapping binding sites for PDF and MetAP when they bind individually at the tunnel exit site, where L22-L32 protein region provides primary anchoring sites for both proteins. In the absence of PDF, trigger factor can access ribosomal tunnel exit when MetAP occupies its primary binding site. Interestingly, however, in the presence of PDF, when MetAP's primary binding site is already engaged, MetAP has a remarkable ability to occupy an alternative binding site adjacent to PDF. Our study, thus, discloses an unexpected mechanism that MetAP adopts for context-specific ribosome association.
リンク	J Mol Biol / PubMed:30753870
手法	EM (単粒子)
解像度	10.5 - 14.2 Å
構造データ	9750 6iy7 EMDB-9750: Cryo-EM density map of E. coli 70S ribosome in complex with peptide deformylase enzyme PDB-6iy7: E. coli peptide deformylase crystal structure fitted into the cryo-EM density map of E. coli 70S ribosome in complex with peptide deformylase 手法: EM (単粒子) / 解像度: 10.5 Å 9752 6iz7 EMDB-9752: Cryo-EM density map of E. coli 70S ribosome in complex with methionine aminopeptidase enzyme PDB-6iz7: E. coli methionine aminopeptidase crystal structure fitted into the cryo-EM density map of E. coli 70S ribosome in complex with methionine aminopeptidase 手法: EM (単粒子) / 解像度: 11.8 Å 9753 6izi EMDB-9753: Cryo-EM density map of peptide deformylase and methionine aminopeptidase bound to the E. coli 70S ribosome PDB-6izi: Crystal structure of E. coli peptide deformylase and methionine aminopeptidase fitted into the cryo-EM density map of the complex 手法: EM (単粒子) / 解像度: 11.8 Å 9759 6j0a EMDB-9759: Cryo-EM density map of methionine aminopeptidase enzyme and chaperone trigger factor bound to the E. coli 70S ribosome PDB-6j0a: Crystal structure of E. coli methionine aminopeptidase enzyme and chaperone trigger factor fitted into the cryo-EM density map of the complex 手法: EM (単粒子) / 解像度: 14.2 Å 9778 6j45 EMDB-9778: Cryo-EM density map of peptide deformylase enzyme and chaperone trigger factor bound to the E. coli 70S ribosome PDB-6j45: Crystal structure of E. coli peptide deformylase enzyme and chaperone trigger factor fitted into the cryo-EM density map of the complex 手法: EM (単粒子) / 解像度: 12.2 Å
由来	escherichia coli k-12 (大腸菌) thermotoga maritima msb8 (テルモトガ・マリティマ) escherichia coli h591 (大腸菌)
キーワード	RIBOSOME (リボソーム) / E. coli 70S ribosome / pepdite deformylase / Nascent polypeptide exit tunnel / Protein biogenesis / Methionine aminopeptidase (メチオニルアミノペプチダーゼ) / Methionine excision / Polypeptide exit tunnel / Peptide deformylase (ペプチドデホルミラーゼ) / Chaperone / Trigger factor / PPIase (プロリルイソメラーゼ)