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Structure paper

TitleStructural Basis for Transcript Elongation Control by NusG Family Universal Regulators.
Journal, issue, pagesCell, Vol. 173, Issue 7, Page 1650-1662.e14, Year 2018
Publish dateJun 14, 2018
AuthorsJin Young Kang / Rachel Anne Mooney / Yuri Nedialkov / Jason Saba / Tatiana V Mishanina / Irina Artsimovitch / Robert Landick / Seth A Darst /
PubMed AbstractNusG/RfaH/Spt5 transcription elongation factors are the only transcription regulators conserved across all life. Bacterial NusG regulates RNA polymerase (RNAP) elongation complexes (ECs) across most ...NusG/RfaH/Spt5 transcription elongation factors are the only transcription regulators conserved across all life. Bacterial NusG regulates RNA polymerase (RNAP) elongation complexes (ECs) across most genes, enhancing elongation by suppressing RNAP backtracking and coordinating ρ-dependent termination and translation. The NusG paralog RfaH engages the EC only at operon polarity suppressor (ops) sites and suppresses both backtrack and hairpin-stabilized pausing. We used single-particle cryoelectron microscopy (cryo-EM) to determine structures of ECs at ops with NusG or RfaH. Both factors chaperone base-pairing of the upstream duplex DNA to suppress backtracking, explaining stimulation of elongation genome-wide. The RfaH-opsEC structure reveals how RfaH confers operon specificity through specific recognition of an ops hairpin in the single-stranded nontemplate DNA and tighter binding to the EC to exclude NusG. Tight EC binding by RfaH sterically blocks the swiveled RNAP conformation necessary for hairpin-stabilized pausing. The universal conservation of NusG/RfaH/Spt5 suggests that the molecular mechanisms uncovered here are widespread.
External linksCell / PubMed:29887376 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 3.7 Å
Structure data

7349

6c6s

EMDB-7349, PDB-6c6s:
CryoEM structure of E.coli RNA polymerase elongation complex bound with RfaH
Method: EM (single particle) / Resolution: 3.7 Å

7350

6c6t

EMDB-7350, PDB-6c6t:
CryoEM structure of E.coli RNA polymerase elongation complex bound with RfaH
Method: EM (single particle) / Resolution: 3.5 Å

7351

6c6u

EMDB-7351, PDB-6c6u:
CryoEM structure of E.coli RNA polymerase elongation complex bound with NusG
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • escherichia coli (E. coli)
  • escherichia coli (strain k12) (bacteria)
Keywordstranscription/dna/rna / RNAP / elongation complex / anti-pausing / ops / TRANSCRIPTION / transcription-dna-rna complex

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