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TitleRNA Polymerase Accommodates a Pause RNA Hairpin by Global Conformational Rearrangements that Prolong Pausing.
Journal, issue, pagesMol Cell, Vol. 69, Issue 5, Page 802-815.e5, Year 2018
Publish dateMar 1, 2018
AuthorsJin Young Kang / Tatiana V Mishanina / Michael J Bellecourt / Rachel Anne Mooney / Seth A Darst / Robert Landick /
PubMed AbstractSequence-specific pausing by RNA polymerase (RNAP) during transcription plays crucial and diverse roles in gene expression. In bacteria, RNA structures are thought to fold within the RNA exit channel ...Sequence-specific pausing by RNA polymerase (RNAP) during transcription plays crucial and diverse roles in gene expression. In bacteria, RNA structures are thought to fold within the RNA exit channel of the RNAP and can increase pause lifetimes significantly. The biophysical mechanism of pausing is uncertain. We used single-particle cryo-EM to determine structures of paused complexes, including a 3.8-Å structure of an RNA hairpin-stabilized, paused RNAP that coordinates RNA folding in the his operon attenuation control region of E. coli. The structures revealed a half-translocated pause state (RNA post-translocated, DNA pre-translocated) that can explain transcriptional pausing and a global conformational change of RNAP that allosterically inhibits trigger loop folding and can explain pause hairpin action. Pause hairpin interactions with the RNAP RNA exit channel suggest how RNAP guides the formation of nascent RNA structures.
External linksMol Cell / PubMed:29499135 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 5.5 Å
Structure data

7002

6asx

EMDB-7002, PDB-6asx:
CryoEM structure of E.coli his pause elongation complex
Method: EM (single particle) / Resolution: 3.8 Å

7103

6bjs

EMDB-7103, PDB-6bjs:
CryoEM structure of E.coli his pause elongation complex without pause hairpin
Method: EM (single particle) / Resolution: 5.5 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • escherichia coli (E. coli)
  • salmonella enterica subsp. enterica serovar typhimurium (bacteria)
  • escherichia coli (strain k12) (bacteria)
Keywordstranscription/dna/rna / DNA-dependent RNA polymerase / TRANSCRIPTION / transcription-dna-rna complex / paused elongation complex

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