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TitleVisualizing virus assembly intermediates inside marine cyanobacteria.
Journal, issue, pagesNature, Vol. 502, Issue 7473, Page 707-710, Year 2013
Publish dateOct 31, 2013
AuthorsWei Dai / Caroline Fu / Desislava Raytcheva / John Flanagan / Htet A Khant / Xiangan Liu / Ryan H Rochat / Cameron Haase-Pettingell / Jacqueline Piret / Steve J Ludtke / Kuniaki Nagayama / Michael F Schmid / Jonathan A King / Wah Chiu /
PubMed AbstractCyanobacteria are photosynthetic organisms responsible for ∼25% of organic carbon fixation on the Earth. These bacteria began to convert solar energy and carbon dioxide into bioenergy and oxygen ...Cyanobacteria are photosynthetic organisms responsible for ∼25% of organic carbon fixation on the Earth. These bacteria began to convert solar energy and carbon dioxide into bioenergy and oxygen more than two billion years ago. Cyanophages, which infect these bacteria, have an important role in regulating the marine ecosystem by controlling cyanobacteria community organization and mediating lateral gene transfer. Here we visualize the maturation process of cyanophage Syn5 inside its host cell, Synechococcus, using Zernike phase contrast electron cryo-tomography (cryoET). This imaging modality yields dramatic enhancement of image contrast over conventional cryoET and thus facilitates the direct identification of subcellular components, including thylakoid membranes, carboxysomes and polyribosomes, as well as phages, inside the congested cytosol of the infected cell. By correlating the structural features and relative abundance of viral progeny within cells at different stages of infection, we identify distinct Syn5 assembly intermediates. Our results indicate that the procapsid releases scaffolding proteins and expands its volume at an early stage of genome packaging. Later in the assembly process, we detected full particles with a tail either with or without an additional horn. The morphogenetic pathway we describe here is highly conserved and was probably established long before that of double-stranded DNA viruses infecting more complex organisms.
External linksNature / PubMed:24107993 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution47.0 - 74.0 Å
Structure data

EMDB-5742:
Zernike Phase Contrast electron cryo-tomography of cyanophage Syn5 assembly intermediates
Method: EM (subtomogram averaging) / Resolution: 68.0 Å

EMDB-5743:
Zernike Phase Contrast electron cryo-tomography of cyanophage Syn5 assembly intermediates
Method: EM (subtomogram averaging) / Resolution: 74.0 Å

EMDB-5744:
Zernike Phase Contrast electron cryo-tomography of cyanophage Syn5 assembly intermediates
Method: EM (subtomogram averaging) / Resolution: 57.0 Å

EMDB-5745:
Zernike Phase Contrast electron cryo-tomography of cyanophage Syn5 assembly intermediates
Method: EM (subtomogram averaging) / Resolution: 47.0 Å

EMDB-5746:
Zernike Phase Contrast electron cryo-tomography of cyanophage Syn5 assembly intermediates
Method: EM (subtomogram averaging) / Resolution: 52.0 Å

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