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TitleStructure of RADX and mechanism for regulation of RAD51 nucleofilaments.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 121, Issue 12, Page e2316491121, Year 2024
Publish dateMar 19, 2024
AuthorsSwati Balakrishnan / Madison Adolph / Miaw-Sheue Tsai / Tae Akizuki / Kaitlyn Gallagher / David Cortez / Walter J Chazin /
PubMed AbstractReplication fork reversal is a fundamental process required for resolution of encounters with DNA damage. A key step in the stabilization and eventual resolution of reversed forks is formation of ...Replication fork reversal is a fundamental process required for resolution of encounters with DNA damage. A key step in the stabilization and eventual resolution of reversed forks is formation of RAD51 nucleoprotein filaments on exposed single strand DNA (ssDNA). To avoid genome instability, RAD51 filaments are tightly controlled by a variety of positive and negative regulators. RADX (RPA-related RAD51-antagonist on the X chromosome) is a recently discovered negative regulator that binds tightly to ssDNA, directly interacts with RAD51, and regulates replication fork reversal and stabilization in a context-dependent manner. Here, we present a structure-based investigation of RADX's mechanism of action. Mass photometry experiments showed that RADX forms multiple oligomeric states in a concentration-dependent manner, with a predominance of trimers in the presence of ssDNA. The structure of RADX, which has no structurally characterized orthologs, was determined ab initio by cryo-electron microscopy (cryo-EM) from maps in the 2 to 4 Å range. The structure reveals the molecular basis for RADX oligomerization and the coupled multi-valent binding of ssDNA binding. The interaction of RADX with RAD51 filaments was imaged by negative stain EM, which showed a RADX oligomer at the end of filaments. Based on these results, we propose a model in which RADX functions by capping and restricting the end of RAD51 filaments.
External linksProc Natl Acad Sci U S A / PubMed:38466836 / PubMed Central
MethodsEM (single particle)
Resolution4.0 Å
Structure data

41940

8u61

EMDB-41940, PDB-8u61:
Human RADX tetramer bound to ssDNA
Method: EM (single particle) / Resolution: 4.0 Å

Source
  • homo sapiens (human)
KeywordsDNA BINDING PROTEIN/DNA / oligomer OB-fold / RAD51 regulator / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex

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