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TitleStructural architecture of the acidic region of the B domain of coagulation factor V.
Journal, issue, pagesJ Thromb Haemost, Vol. 22, Issue 3, Page 709-714, Year 2024
Publish dateNov 23, 2023
AuthorsBassem M Mohammed / Katherine Basore / Brock Summers / Leslie A Pelc / Enrico Di Cera /
PubMed AbstractBACKGROUND: Coagulation factor (F)V features an A1-A2-B-A3-C1-C2 domain organization and functions as the inactive precursor of FVa, a component of the prothrombinase complex required for rapid ...BACKGROUND: Coagulation factor (F)V features an A1-A2-B-A3-C1-C2 domain organization and functions as the inactive precursor of FVa, a component of the prothrombinase complex required for rapid thrombin generation in the penultimate step of the coagulation cascade. An intramolecular interaction within the large B domain (residues 710-1545) involves the basic region (BR, residues 963-1008) and acidic region (AR, residues 1493-1537) and locks FV in its inactive state. However, structural information on this important regulatory interaction or on the separate architecture of the AR and BR remains elusive due to conformational disorder of the B domain.
OBJECTIVES: To reveal the structure of the BR-AR interaction or of its separate components.
METHODS: The structure of FV is solved by cryogenic electron microscopy.
RESULTS: A new 3.05 Å resolution cryogenic electron microscopy structure of FV confirms the overall organization of the A and C domains but resolves the segment 1507 to 1545 within a largely disordered B domain. The segment contains most of the AR and is organized as recently reported in FV short, a spliced variant of FV with a significantly shorter and less disordered B domain.
CONCLUSION: The similar architecture of the AR in FV and FV short provides structural context for physiologically important interactions of this region with the BR in FV and with the basic C-terminal end of tissue factor pathway inhibitor α in FV short.
External linksJ Thromb Haemost / PubMed:38007061 / PubMed Central
MethodsEM (single particle)
Resolution3.05 - 3.24 Å
Structure data

EMDB-41400: Structural architecture of the acidic region of the B domain of coagulation factor V
Method: EM (single particle) / Resolution: 3.05 Å

EMDB-41401: Structural architecture of the acidic region of the B domain of coagulation factor V
Method: EM (single particle) / Resolution: 3.09 Å

EMDB-41402: Structural architecture of the acidic region of the B domain of coagulation factor V
Method: EM (single particle) / Resolution: 3.09 Å

EMDB-41403: Structural architecture of the acidic region of the B domain of coagulation factor V
Method: EM (single particle) / Resolution: 3.08 Å

EMDB-41407: Structural architecture of the acidic region of the B domain of coagulation factor V
Method: EM (single particle) / Resolution: 3.14 Å

EMDB-41408: Structural architecture of the acidic region of the B domain of coagulation factor V
Method: EM (single particle) / Resolution: 3.24 Å

41411

8tn9

EMDB-41411, PDB-8tn9:
Structural architecture of the acidic region of the B domain of coagulation factor V
Method: EM (single particle) / Resolution: 3.05 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • homo sapiens (human)
KeywordsBLOOD CLOTTING / Coagulation / Pro-cofactor / Factor V / B Domain / Acidic Region

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