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TitleTopology and structure of an engineered human cohesin complex bound to Pds5B.
Journal, issue, pagesNat Commun, Vol. 7, Page 12523, Year 2016
Publish dateAug 23, 2016
AuthorsMichael T Hons / Pim J Huis In 't Veld / Jan Kaesler / Pascaline Rombaut / Alexander Schleiffer / Franz Herzog / Holger Stark / Jan-Michael Peters /
PubMed AbstractThe cohesin subunits Smc1, Smc3 and Scc1 form large tripartite rings which mediate sister chromatid cohesion and chromatin structure. These are thought to entrap DNA with the help of the associated ...The cohesin subunits Smc1, Smc3 and Scc1 form large tripartite rings which mediate sister chromatid cohesion and chromatin structure. These are thought to entrap DNA with the help of the associated proteins SA1/2 and Pds5A/B. Structural information is available for parts of cohesin, but analyses of entire cohesin complexes are limited by their flexibility. Here we generated a more rigid 'bonsai' cohesin by truncating the coiled coils of Smc1 and Smc3 and used single-particle electron microscopy, chemical crosslinking-mass spectrometry and in silico modelling to generate three-dimensional models of cohesin bound to Pds5B. The HEAT-repeat protein Pds5B forms a curved structure around the nucleotide-binding domains of Smc1 and Smc3 and bridges the Smc3-Scc1 and SA1-Scc1 interfaces. These results indicate that Pds5B forms an integral part of the cohesin ring by contacting all other cohesin subunits, a property that may reflect the complex role of Pds5 proteins in controlling cohesin-DNA interactions.
External linksNat Commun / PubMed:27549742 / PubMed Central
MethodsEM (single particle)
Resolution28.0 - 35.0 Å
Structure data

EMDB-4029:
An engineered human cohesin complex bound to Pds5B
Method: EM (single particle) / Resolution: 31.0 Å

EMDB-4030:
An engineered human cohesin complex
Method: EM (single particle) / Resolution: 28.0 Å

EMDB-4031:
An engineered trimeric human cohesin complex (-SA1) bound to Pds5B
Method: EM (single particle) / Resolution: 35.0 Å

Source
  • Homo sapiens (human)

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