EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility.
ジャーナル・号・ページ	Cell, Vol. 186, Issue 13, Page 2897-2910.e19, Year 2023
掲載日	2023年6月22日
著者	Lunni Zhou / Haobin Liu / Siyu Liu / Xiaoyu Yang / Yue Dong / Yun Pan / Zhuang Xiao / Beihong Zheng / Yan Sun / Pengyu Huang / Xixi Zhang / Jin Hu / Rui Sun / Shan Feng / Yi Zhu / Mingxi Liu / Miao Gui / Jianping Wu /
PubMed 要旨	Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules (DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using cryo-electron ...Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules (DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using cryo-electron microscopy (cryo-EM) and artificial intelligence (AI)-based modeling, we determined the structures of mouse and human sperm DMTs and built an atomic model of the 48-nm repeat of the mouse sperm DMT. Our analysis revealed 47 DMT-associated proteins, including 45 microtubule inner proteins (MIPs). We identified 10 sperm-specific MIPs, including seven classes of Tektin5 in the lumen of the A tubule and FAM166 family members that bind the intra-tubulin interfaces. Interestingly, the human sperm DMT lacks some MIPs compared with the mouse sperm DMT. We also discovered variants in 10 distinct MIPs associated with a subtype of asthenozoospermia characterized by impaired sperm motility without evident morphological abnormalities. Our study highlights the conservation and tissue/species specificity of DMTs and expands the genetic spectrum of male infertility.
リンク	Cell / PubMed:37295417
手法	EM (単粒子)
解像度	3.07 - 4.34 Å
構造データ	EMDB-35810: Cryo-EM structure of the 48-nm repeat doublet microtubule from human sperm 手法: EM (単粒子) / 解像度: 4.3 Å 35823 8iyj EMDB-35823, PDB-8iyj: Cryo-EM structure of the 48-nm repeat doublet microtubule from mouse sperm 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-36458: Local map of the mouse sperm doublet microtubule (Middel01) 手法: EM (単粒子) / 解像度: 3.17 Å EMDB-36498: Overall consensus map of the mouse sperm doublet microtubule 手法: EM (単粒子) / 解像度: 3.52 Å EMDB-36499: Local map of the mouse sperm doublet microtubule (Lower01) 手法: EM (単粒子) / 解像度: 3.26 Å EMDB-36500: Local map of the mouse sperm doublet microtubule (Upper10) 手法: EM (単粒子) / 解像度: 3.44 Å EMDB-36501: Local map of the mouse sperm doublet microtubule (Middle02) 手法: EM (単粒子) / 解像度: 3.28 Å EMDB-36502: Local map of the mouse sperm doublet microtubule (Lower02) 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-36503: Local map of the mouse sperm doublet microtubule (Upper09) 手法: EM (単粒子) / 解像度: 3.87 Å EMDB-36504: Local map of the mouse sperm doublet microtubule (Middle03) 手法: EM (単粒子) / 解像度: 3.35 Å EMDB-36509: Local map of the mouse sperm doublet microtubule (Lower03) 手法: EM (単粒子) / 解像度: 3.44 Å EMDB-36510: Local map of the mouse sperm doublet microtubule (Middle04) 手法: EM (単粒子) / 解像度: 3.37 Å EMDB-36511: Local map of the mouse sperm doublet microtubule (Upper08) 手法: EM (単粒子) / 解像度: 3.67 Å EMDB-36513: Local map of the mouse sperm doublet microtubule (Lower04) 手法: EM (単粒子) / 解像度: 3.43 Å EMDB-36514: Local map of the mouse sperm doublet microtubule (Upper07) 手法: EM (単粒子) / 解像度: 3.62 Å EMDB-36515: Local map of the mouse sperm doublet microtubule (Middle05) 手法: EM (単粒子) / 解像度: 3.24 Å EMDB-36516: Local map of the mouse sperm doublet microtubule (Lower05) 手法: EM (単粒子) / 解像度: 3.35 Å EMDB-36517: Local map of the mouse sperm doublet microtubule (Upper06) 手法: EM (単粒子) / 解像度: 3.25 Å EMDB-36518: Local map of the mouse sperm doublet microtubule (Middle06) 手法: EM (単粒子) / 解像度: 3.07 Å EMDB-36519: Local map of the mouse sperm doublet microtubule (Lower06) 手法: EM (単粒子) / 解像度: 3.21 Å EMDB-36520: Local map of the mouse sperm doublet microtubule (Upper05) 手法: EM (単粒子) / 解像度: 3.36 Å EMDB-36521: Local map of the mouse sperm doublet microtubule (Middle07) 手法: EM (単粒子) / 解像度: 3.52 Å EMDB-36523: Local map of the mouse sperm doublet microtubule (Upper04) 手法: EM (単粒子) / 解像度: 3.44 Å EMDB-36524: Local map of the mouse sperm doublet microtubule (Middle08) 手法: EM (単粒子) / 解像度: 3.59 Å EMDB-36525: Local map of the mouse sperm doublet microtubule (Lower07) 手法: EM (単粒子) / 解像度: 3.65 Å EMDB-36526: Local map of the mouse sperm doublet microtubule (Upper03) 手法: EM (単粒子) / 解像度: 3.45 Å EMDB-36527: Local map of the mouse sperm doublet microtubule (Middle09) 手法: EM (単粒子) / 解像度: 3.79 Å EMDB-36528: Local map of the mouse sperm doublet microtubule (Lower08) 手法: EM (単粒子) / 解像度: 3.65 Å EMDB-36529: Local map of the mouse sperm doublet microtubule (Upper02) 手法: EM (単粒子) / 解像度: 3.43 Å EMDB-36530: Local map of the mouse sperm doublet microtubule (Middle10) 手法: EM (単粒子) / 解像度: 3.55 Å EMDB-36531: Local map of the mouse sperm doublet microtubule (Lower09) 手法: EM (単粒子) / 解像度: 3.89 Å EMDB-36532: Local map of the mouse sperm doublet microtubule (Upper01) 手法: EM (単粒子) / 解像度: 3.33 Å EMDB-36533: Local map of the mouse sperm doublet microtubule (Lower10) 手法: EM (単粒子) / 解像度: 3.53 Å EMDB-36534: Overall consensus map of the human sperm doublet microtubule 手法: EM (単粒子) / 解像度: 4.34 Å EMDB-36536: Local map of the human sperm doublet microtubule (Lower01) 手法: EM (単粒子) / 解像度: 4.07 Å EMDB-36537: Local map of the human sperm doublet microtubule (Lower02) 手法: EM (単粒子) / 解像度: 4.06 Å EMDB-36538: Local map of the human sperm doublet microtubule (Lower03) 手法: EM (単粒子) / 解像度: 4.15 Å EMDB-36539: Local map of the human sperm doublet microtubule (Lower04) 手法: EM (単粒子) / 解像度: 4.12 Å EMDB-36540: Local map of the human sperm doublet microtubule (Lower05) 手法: EM (単粒子) / 解像度: 3.91 Å EMDB-36542: Local map of the human sperm doublet microtubule (Lower06) 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-36543: Local map of the human sperm doublet microtubule (Lower07) 手法: EM (単粒子) / 解像度: 4.06 Å EMDB-36544: Local map of the human sperm doublet microtubule (Lower08) 手法: EM (単粒子) / 解像度: 4.27 Å EMDB-36545: Local map of the human sperm doublet microtubule (Lower09) 手法: EM (単粒子) / 解像度: 4.29 Å EMDB-36546: Local map of the human sperm doublet microtubule (Lower10) 手法: EM (単粒子) / 解像度: 3.92 Å EMDB-36547: Local map of the human sperm doublet microtubule (Middle01) 手法: EM (単粒子) / 解像度: 3.77 Å EMDB-36549: Local map of the human sperm doublet microtubule (Middle02) 手法: EM (単粒子) / 解像度: 3.92 Å EMDB-36550: Local map of the human sperm doublet microtubule (Middle03) 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-36551: Local map of the human sperm doublet microtubule (Middle04) 手法: EM (単粒子) / 解像度: 3.93 Å EMDB-36552: Local map of the human sperm doublet microtubule (Middle05) 手法: EM (単粒子) / 解像度: 3.92 Å EMDB-36553: Local map of the human sperm doublet microtubule (Middle06) 手法: EM (単粒子) / 解像度: 3.56 Å EMDB-36554: Local map of the human sperm doublet microtubule (Middle07) 手法: EM (単粒子) / 解像度: 3.98 Å EMDB-36555: Local map of the human sperm doublet microtubule (Middle08) 手法: EM (単粒子) / 解像度: 4.14 Å EMDB-36556: Local map of the human sperm doublet microtubule (Middle09) 手法: EM (単粒子) / 解像度: 4.25 Å EMDB-36557: Local map of the human sperm doublet microtubule (Middle10) 手法: EM (単粒子) / 解像度: 3.95 Å EMDB-36558: Local map of the human sperm doublet microtubule (Upper01) 手法: EM (単粒子) / 解像度: 3.83 Å EMDB-36559: Local map of the human sperm doublet microtubule (Upper02) 手法: EM (単粒子) / 解像度: 3.94 Å EMDB-36560: Local map of the human sperm doublet microtubule (Upper03) 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-36561: Local map of the human sperm doublet microtubule (Upper04) 手法: EM (単粒子) / 解像度: 4.07 Å EMDB-36562: Local map of the human sperm doublet microtubule (Upper05) 手法: EM (単粒子) / 解像度: 3.92 Å EMDB-36564: Local map of the human sperm doublet microtubule (Upper06) 手法: EM (単粒子) / 解像度: 3.71 Å EMDB-36565: Local map of the human sperm doublet microtubule (Upper07) 手法: EM (単粒子) / 解像度: 4.06 Å EMDB-36566: Local map of the human sperm doublet microtubule (Upper08) 手法: EM (単粒子) / 解像度: 4.21 Å EMDB-36567: Local map of the human sperm doublet microtubule (Upper09) 手法: EM (単粒子) / 解像度: 4.33 Å EMDB-36568: Local map of the human sperm doublet microtubule (Upper10) 手法: EM (単粒子) / 解像度: 4.04 Å EMDB-36580: Local map of the mouse sperm doublet microtubule (Upper_ODF3A) 手法: EM (単粒子) / 解像度: 3.43 Å EMDB-36581: Local map of the mouse sperm doublet microtubule (Middle_ODF3A) 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-36582: Local map of the mouse sperm doublet microtubule (Lower_ODF3A) 手法: EM (単粒子) / 解像度: 3.48 Å EMDB-36583: Local map of the mouse sperm doublet microtubule (Middle_A13) 手法: EM (単粒子) / 解像度: 3.21 Å EMDB-36584: Local map of the mouse sperm doublet microtubule (Upper_A13) 手法: EM (単粒子) / 解像度: 3.27 Å EMDB-36585: Local map of the mouse sperm doublet microtubule (TSSK6) 手法: EM (単粒子) / 解像度: 3.52 Å EMDB-36586: Local map of the mouse sperm doublet microtubule (A07_A08) 手法: EM (単粒子) / 解像度: 3.65 Å EMDB-36587: Local map of the mouse sperm doublet microtubule (DUSP21_TEX37) 手法: EM (単粒子) / 解像度: 3.22 Å EMDB-36588: Local map of the mouse sperm doublet microtubule (A09_A10) 手法: EM (単粒子) / 解像度: 3.41 Å EMDB-36591: Local map of the mouse sperm doublet microtubule (A13_A01_A02) 手法: EM (単粒子) / 解像度: 3.23 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸
由来	Homo sapiens (ヒト) mus musculus (ハツカネズミ) house mouse (ハツカネズミ)
キーワード	STRUCTURAL PROTEIN (タンパク質) / doublet microtubule