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TitleRegulatory dynamics of the higher-plant PSI-LHCI supercomplex during state transitions.
Journal, issue, pagesMol Plant, Vol. 16, Issue 12, Page 1937-1950, Year 2023
Publish dateDec 4, 2023
AuthorsJianghao Wu / Shuaijiabin Chen / Chao Wang / Weijun Lin / Chao Huang / Chengxu Fan / Dexian Han / Dandan Lu / Xiumei Xu / SenFang Sui / Lixin Zhang /
PubMed AbstractState transition is a fundamental light acclimation mechanism of photosynthetic organisms in response to the environmental light conditions. This process rebalances the excitation energy between ...State transition is a fundamental light acclimation mechanism of photosynthetic organisms in response to the environmental light conditions. This process rebalances the excitation energy between photosystem I (PSI) and photosystem II through regulated reversible binding of the light-harvesting complex II (LHCII) to PSI. However, the structural reorganization of PSI-LHCI, the dynamic binding of LHCII, and the regulatory mechanisms underlying state transitions are less understood in higher plants. In this study, using cryoelectron microscopy we resolved the structures of PSI-LHCI in both state 1 (PSI-LHCI-ST1) and state 2 (PSI-LHCI-LHCII-ST2) from Arabidopsis thaliana. Combined genetic and functional analyses revealed novel contacts between Lhcb1 and PsaK that further enhanced the binding of the LHCII trimer to the PSI core with the known interactions between phosphorylated Lhcb2 and the PsaL/PsaH/PsaO subunits. Specifically, PsaO was absent in the PSI-LHCI-ST1 supercomplex but present in the PSI-LHCI-LHCII-ST2 supercomplex, in which the PsaL/PsaK/PsaA subunits undergo several conformational changes to strengthen the binding of PsaO in ST2. Furthermore, the PSI-LHCI module adopts a more compact configuration with shorter Mg-to-Mg distances between the chlorophylls, which may enhance the energy transfer efficiency from the peripheral antenna to the PSI core in ST2. Collectively, our work provides novel structural and functional insights into the mechanisms of light acclimation during state transitions in higher plants.
External linksMol Plant / PubMed:37936349
MethodsEM (single particle)
Resolution2.79 - 3.53 Å
Structure data

EMDB-36020: Cryo-EM structure of the Arabidopsis thaliana photosystem I(PSI-LHCII-ST2)
Method: EM (single particle) / Resolution: 2.79 Å

36021

8j6z

EMDB-36021, PDB-8j6z:
Cryo-EM structure of the Arabidopsis thaliana photosystem I(PSI-LHCII-ST2)
Method: EM (single particle) / Resolution: 2.79 Å

EMDB-36022: LHCI of PSI-LHCII from Arabidopsis thaliana(state2)
Method: EM (single particle) / Resolution: 3.22 Å

EMDB-36023: LHCII of PSI-LHCII from Arabidopsis thaliana(state2)
Method: EM (single particle) / Resolution: 3.15 Å

EMDB-36026: Cryo-EM structure of the Arabidopsis thaliana photosystem I in state 2(PSI-ST2)
Method: EM (single particle) / Resolution: 2.84 Å

EMDB-36032: LHCI of PSI from Arabidopsis thaliana in state 2 (PSI-ST2)
Method: EM (single particle) / Resolution: 3.22 Å

EMDB-36033: Cryo-EM structure of the Arabidopsis thaliana PSI in state 1 (PSI-ST1)
Method: EM (single particle) / Resolution: 3.06 Å

EMDB-36035: LHCI of PSI from Arabidopsis thaliana in state 1 (PSI-ST1)
Method: EM (single particle) / Resolution: 3.53 Å

36036

8j7a

EMDB-36036, PDB-8j7a:
Coordinates of Cryo-EM structure of the Arabidopsis thaliana PSI in state 1 (PSI-ST1)
Method: EM (single particle) / Resolution: 3.06 Å

36037

8j7b

EMDB-36037, PDB-8j7b:
Coordinates of Cryo-EM structure of the Arabidopsis thaliana PSI in state 2 (PSI-ST2)
Method: EM (single particle) / Resolution: 3.22 Å

Chemicals

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Violaxanthin

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-CL0:
CHLOROPHYLL A ISOMER / Chlorophyll a

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

Source
  • arabidopsis thaliana (thale cress)
KeywordsPHOTOSYNTHESIS / photosystem I / PSI / CELL CYCLE / photosystem / PLANT PROTEIN

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