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Structure paper

TitleStructural insights into human brain-gut peptide cholecystokinin receptors.
Journal, issue, pagesCell Discov, Vol. 8, Issue 1, Page 55, Year 2022
Publish dateJun 7, 2022
AuthorsYu Ding / Huibing Zhang / Yu-Ying Liao / Li-Nan Chen / Su-Yu Ji / Jiao Qin / Chunyou Mao / Dan-Dan Shen / Lin Lin / Hao Wang / Yan Zhang / Xiao-Ming Li /
PubMed AbstractThe intestinal hormone and neuromodulator cholecystokinin (CCK) receptors CCK1R and CCK2R act as a signaling hub in brain-gut axis, mediating digestion, emotion, and memory regulation. CCK receptors ...The intestinal hormone and neuromodulator cholecystokinin (CCK) receptors CCK1R and CCK2R act as a signaling hub in brain-gut axis, mediating digestion, emotion, and memory regulation. CCK receptors exhibit distinct preferences for ligands in different posttranslational modification (PTM) states. CCK1R couples to G and G, whereas CCK2R primarily couples to G. Here we report the cryo-electron microscopy (cryo-EM) structures of CCK1R-G signaling complexes liganded either by sulfated cholecystokinin octapeptide (CCK-8) or a CCK1R-selective small-molecule SR146131, and CCK2R-G complexes stabilized by either sulfated CCK-8 or a CCK2R-selective ligand gastrin-17. Our structures reveal a location-conserved yet charge-distinct pocket discriminating the effects of ligand PTM states on receptor subtype preference, the unique pocket topology underlying selectivity of SR146131 and gastrin-17, the conformational changes in receptor activation, and key residues contributing to G protein subtype specificity, providing multiple structural templates for drug design targeting the brain-gut axis.
External linksCell Discov / PubMed:35672283 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.2 Å
Structure data

33359

7xou

EMDB-33359, PDB-7xou:
Structural insights into human brain gut peptide cholecystokinin receptors
Method: EM (single particle) / Resolution: 3.2 Å

33360

7xov

EMDB-33360, PDB-7xov:
Structural insights into human brain gut peptide cholecystokinin receptors
Method: EM (single particle) / Resolution: 3.0 Å

33361

7xow

EMDB-33361, PDB-7xow:
Structural insights into human brain gut peptide cholecystokinin receptors
Method: EM (single particle) / Resolution: 3.1 Å

35297

8ia7

EMDB-35297, PDB-8ia7:
Structural insights into human brain gut peptide cholecystokinin receptors
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-IA1:
2-[2-[[4-(4-chloranyl-2,5-dimethoxy-phenyl)-5-(2-cyclohexylethyl)-1,3-thiazol-2-yl]carbamoyl]-5,7-dimethyl-indol-1-yl]ethanoic acid

ChemComp-HOH:
WATER / Water

Source
  • homo sapiens (human)
  • synthetic construct (others)
  • mus musculus (house mouse)
KeywordsNEUROPEPTIDE / brain gut peptide receptor Class A G-protein-coupled receptor

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