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TitleCryo-Electron Tomography of Toxoplasma gondii Indicates That the Conoid Fiber May Be Derived from Microtubules.
Journal, issue, pagesAdv Sci (Weinh), Vol. 10, Issue 14, Page e2206595, Year 2023
Publish dateFeb 25, 2023
AuthorsZhixun Li / Wenjing Du / Jiong Yang / De-Hua Lai / Zhao-Rong Lun / Qiang Guo /
PubMed AbstractToxoplasma gondii (T. gondii) is the causative agent of toxoplasmosis and can infect numerous warm-blooded animals. An improved understanding of the fine structure of this parasite can help elucidate ...Toxoplasma gondii (T. gondii) is the causative agent of toxoplasmosis and can infect numerous warm-blooded animals. An improved understanding of the fine structure of this parasite can help elucidate its replication mechanism. Previous studies have resolved the ultrastructure of the cytoskeleton using purified samples, which eliminates their cellular context. Here the application of cryo-electron tomography to visualize T. gondii tachyzoites in their native state is reported. The fine structure and cellular distribution of the cytoskeleton are resolved and analyzed at nanometer resolution. Additionally, the tachyzoite structural characteristics are annotated during its endodyogeny for the first time. By comparing the structural features in mature tachyzoites and their daughter buds, it is proposed that the conoid fiber of the Apicomplexa originates from microtubules. This work represents the detailed molecular anatomy of T. gondii, particularly during the budding replication stage of tachyzoite, and provides a reference for further studies of this fascinating organism.
External linksAdv Sci (Weinh) / PubMed:36840635 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution28.0 Å
Structure data

EMDB-35096: The in situ structure of conoid fiber from Toxoplasma gondii tachyzoite
Method: EM (subtomogram averaging) / Resolution: 28.0 Å

EMDB-35097: Tomogram of apical part of Toxoplasma gondii tachyzoite
Method: EM (tomography)

EMDB-35098: Tomogram of daughter buds in mature Toxoplasma gondii tachyzoite
Method: EM (tomography)

Source
  • Toxoplasma gondii RH (eukaryote)

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