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TitleLigand recognition mechanism of the human relaxin family peptide receptor 4 (RXFP4).
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 492, Year 2023
Publish dateJan 30, 2023
AuthorsYan Chen / Qingtong Zhou / Jiang Wang / Youwei Xu / Yun Wang / Jiahui Yan / Yibing Wang / Qi Zhu / Fenghui Zhao / Chenghao Li / Chuan-Wei Chen / Xiaoqing Cai / Ross A D Bathgate / Chun Shen / H Eric Xu / Dehua Yang / Hong Liu / Ming-Wei Wang /
PubMed AbstractMembers of the insulin superfamily regulate pleiotropic biological processes through two types of target-specific but structurally conserved peptides, insulin/insulin-like growth factors and ...Members of the insulin superfamily regulate pleiotropic biological processes through two types of target-specific but structurally conserved peptides, insulin/insulin-like growth factors and relaxin/insulin-like peptides. The latter bind to the human relaxin family peptide receptors (RXFPs). Here, we report three cryo-electron microscopy structures of RXFP4-G protein complexes in the presence of the endogenous ligand insulin-like peptide 5 (INSL5) or one of the two small molecule agonists, compound 4 and DC591053. The B chain of INSL5 adopts a single α-helix that penetrates into the orthosteric pocket, while the A chain sits above the orthosteric pocket, revealing a peptide-binding mode previously unknown. Together with mutagenesis and functional analyses, the key determinants responsible for the peptidomimetic agonism and subtype selectivity were identified. Our findings not only provide insights into ligand recognition and subtype selectivity among class A G protein-coupled receptors, but also expand the knowledge of signaling mechanisms in the insulin superfamily.
External linksNat Commun / PubMed:36717591 / PubMed Central
MethodsEM (single particle)
Resolution2.75 - 3.19 Å
Structure data

33871

7yj4

EMDB-33871, PDB-7yj4:
Cryo-EM structure of the INSL5-bound human relaxin family peptidereceptor 4 (RXFP4)-Gi complex
Method: EM (single particle) / Resolution: 3.19 Å

33888

7yk6

EMDB-33888, PDB-7yk6:
Cryo-EM structure of the compound 4-bound human relaxin family peptide receptor 4 (RXFP4)-Gi complex
Method: EM (single particle) / Resolution: 3.03 Å

33889

7yk7

EMDB-33889, PDB-7yk7:
Cryo-EM structure of the DC591053-bound human relaxin family peptide receptor 4 (RXFP4)-Gi complex
Method: EM (single particle) / Resolution: 2.75 Å

Chemicals

ChemComp-IYF:
1-[2-(4-chlorophenyl)ethyl]-3-[(7-ethyl-5-oxidanyl-1H-indol-3-yl)methylideneamino]guanidine

ChemComp-IYM:
[(1S)-7-ethoxy-6-methoxy-1-[2-(5-methoxy-1H-indol-3-yl)ethyl]-3,4-dihydro-1H-isoquinolin-2-yl]-morpholin-4-yl-methanone

Source
  • homo sapiens (human)
  • synthetic construct (others)
  • bos taurus (cattle)
KeywordsSTRUCTURAL PROTEIN / human relaxin family peptide receptor 4 / G protein-coupled receptor / ligand recognition

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