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TitleStructures of the ADGRG2-G complex in apo and ligand-bound forms.
Journal, issue, pagesNat Chem Biol, Vol. 18, Issue 11, Page 1196-1203, Year 2022
Publish dateAug 18, 2022
AuthorsHui Lin / Peng Xiao / Rui-Qian Bu / Shengchao Guo / Zhao Yang / Daopeng Yuan / Zhong-Liang Zhu / Chuan-Xin Zhang / Qing-Tao He / Chao Zhang / Yu-Qi Ping / Ru-Jia Zhao / Chuan-Shun Ma / Chang-Hao Liu / Xiao-Ning Zhang / Dan Jiang / Shaohui Huang / Yue-Tong Xi / Dao-Lai Zhang / Chen-Yang Xue / Bai-Sheng Yang / Jian-Yuan Li / Hao-Cheng Lin / Xu-Hui Zeng / Han Zhao / Wen-Ming Xu / Fan Yi / Zhongmin Liu / Jin-Peng Sun / Xiao Yu /
PubMed AbstractAdhesion G protein-coupled receptors are elusive in terms of their structural information and ligands. Here, we solved the cryogenic-electron microscopy (cryo-EM) structure of apo-ADGRG2, an ...Adhesion G protein-coupled receptors are elusive in terms of their structural information and ligands. Here, we solved the cryogenic-electron microscopy (cryo-EM) structure of apo-ADGRG2, an essential membrane receptor for maintaining male fertility, in complex with a G trimer. Whereas the formations of two kinks were determinants of the active state, identification of a potential ligand-binding pocket in ADGRG2 facilitated the screening and identification of dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate and deoxycorticosterone as potential ligands of ADGRG2. The cryo-EM structures of DHEA-ADGRG2-G provided interaction details for DHEA within the seven transmembrane domains of ADGRG2. Collectively, our data provide a structural basis for the activation and signaling of ADGRG2, as well as characterization of steroid hormones as ADGRG2 ligands, which might be used as useful tools for further functional studies of the orphan ADGRG2.
External linksNat Chem Biol / PubMed:35982227
MethodsEM (single particle)
Resolution2.4 - 2.9 Å
Structure data

33248

7xkd

EMDB-33248, PDB-7xkd:
Cryo-EM structure of DHEA-ADGRG2-BT-Gs complex
Method: EM (single particle) / Resolution: 2.4 Å

33249

7xke

EMDB-33249, PDB-7xke:
Cryo-EM structure of DHEA-ADGRG2-FL-Gs complex
Method: EM (single particle) / Resolution: 2.9 Å

33250

7xkf

EMDB-33250, PDB-7xkf:
Cryo-EM structure of DHEA-ADGRG2-BT-Gs complex at lower state
Method: EM (single particle) / Resolution: 2.4 Å

Chemicals

ChemComp-AND:
3-BETA-HYDROXY-5-ANDROSTEN-17-ONE / hormone*YM / Dehydroepiandrosterone

ChemComp-HOH:
WATER / Water

Source
  • homo sapiens (human)
  • camelus bactrianus (Bactrian camel)
  • mus musculus (house mouse)
KeywordsMEMBRANE PROTEIN / ADGRG2 / GPCR / complex

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