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TitleStructural insights into human CCAN complex assembled onto DNA.
Journal, issue, pagesCell Discov, Vol. 8, Issue 1, Page 90, Year 2022
Publish dateSep 9, 2022
AuthorsTian Tian / Lili Chen / Zhen Dou / Zhisen Yang / Xinjiao Gao / Xiao Yuan / Chengliang Wang / Ran Liu / Zuojun Shen / Ping Gui / Maikun Teng / Xianlei Meng / Donald L Hill / Lin Li / Xuan Zhang / Xing Liu / Linfeng Sun / Jianye Zang / Xuebiao Yao /
PubMed AbstractIn mitosis, accurate chromosome segregation depends on kinetochores that connect centromeric chromatin to spindle microtubules. The centromeres of budding yeast, which are relatively simple, are ...In mitosis, accurate chromosome segregation depends on kinetochores that connect centromeric chromatin to spindle microtubules. The centromeres of budding yeast, which are relatively simple, are connected to individual microtubules via a kinetochore constitutive centromere associated network (CCAN). However, the complex centromeres of human chromosomes comprise millions of DNA base pairs and attach to multiple microtubules. Here, by use of cryo-electron microscopy and functional analyses, we reveal the molecular basis of how human CCAN interacts with duplex DNA and facilitates accurate chromosome segregation. The overall structure relates to the cooperative interactions and interdependency of the constituent sub-complexes of the CCAN. The duplex DNA is topologically entrapped by human CCAN. Further, CENP-N does not bind to the RG-loop of CENP-A but to DNA in the CCAN complex. The DNA binding activity is essential for CENP-LN localization to centromere and chromosome segregation during mitosis. Thus, these analyses provide new insights into mechanisms of action underlying kinetochore assembly and function in mitosis.
External linksCell Discov / PubMed:36085283 / PubMed Central
MethodsEM (single particle)
Resolution3.29 - 3.71 Å
Structure data

33196

7xhn

EMDB-33196, PDB-7xhn:
Structure of human inner kinetochore CCAN-DNA complex
Method: EM (single particle) / Resolution: 3.71 Å

33197

7xho

EMDB-33197, PDB-7xho:
Structure of human inner kinetochore CCAN complex
Method: EM (single particle) / Resolution: 3.29 Å

Source
  • homo sapiens (human)
KeywordsCELL CYCLE

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