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TitleStructural insights into blue-green light utilization by marine green algal light harvesting complex II at 2.78 Å.
Journal, issue, pagesBBA Adv, Vol. 2, Page 100064, Year 2022
Publish dateNov 11, 2022
AuthorsSoichiro Seki / Tetsuko Nakaniwa / Pablo Castro-Hartmann / Kasim Sader / Akihiro Kawamoto / Hideaki Tanaka / Pu Qian / Genji Kurisu / Ritsuko Fujii /
PubMed AbstractLight-harvesting complex II (LHCII) present in plants and green algae absorbs solar energy to promote photochemical reactions. A marine green macroalga, , exhibits the unique characteristic of ...Light-harvesting complex II (LHCII) present in plants and green algae absorbs solar energy to promote photochemical reactions. A marine green macroalga, , exhibits the unique characteristic of absorbing blue-green light from the sun during photochemical reactions while being underwater owing to the presence of pigment-altered LHCII called siphonaxanthin-chlorophyll binding protein (SCP). In this study, we determined the structure of SCP at a resolution of 2.78 Å using cryogenic electron microscopy. SCP has a trimeric structure, wherein each monomer containing two lutein and two chlorophyll molecules in the plant-type LHCII are replaced by siphonaxanthin and its ester and two chlorophyll molecules, respectively. Siphonaxanthin occupies the binding site in SCP having a polarity in the trimeric inner core, and exhibits a distorted conjugated chain comprising a carbonyl group hydrogen bonded to a cysteine residue of apoprotein. These features suggest that the siphonaxanthin molecule is responsible for the characteristic green absorption of SCP. The replaced chlorophyll molecules extend the region of the stromal side chlorophyll cluster, spanning two adjacent monomers.
External linksBBA Adv / PubMed:37082593 / PubMed Central
MethodsEM (single particle)
Resolution2.8 Å
Structure data

32588

7wlm

EMDB-32588, PDB-7wlm:
The Cryo-EM structure of siphonaxanthin chlorophyll a/b type light-harvesting complex II
Method: EM (single particle) / Resolution: 2.8 Å

Chemicals

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

ChemComp-0UR:
Siphonein

ChemComp-0IE:
Siphonaxanthin

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-HOH:
WATER / Water

Source
  • codium fragile (dead man's fingers)
KeywordsPHOTOSYNTHESIS / light-harvesting complex II / siphonaxanthin / Chlorophyll b replacement / blue-green utilization

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