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TitleStructures and mechanisms of the Arabidopsis auxin transporter PIN3.
Journal, issue, pagesNature, Vol. 609, Issue 7927, Page 616-621, Year 2022
Publish dateAug 2, 2022
AuthorsNannan Su / Aiqin Zhu / Xin Tao / Zhong Jie Ding / Shenghai Chang / Fan Ye / Yan Zhang / Cheng Zhao / Qian Chen / Jiangqin Wang / Chen Yu Zhou / Yirong Guo / Shasha Jiao / Sufen Zhang / Han Wen / Lixin Ma / Sheng Ye / Shao Jian Zheng / Fan Yang / Shan Wu / Jiangtao Guo /
PubMed AbstractThe PIN-FORMED (PIN) protein family of auxin transporters mediates polar auxin transport and has crucial roles in plant growth and development. Here we present cryo-electron microscopy structures of ...The PIN-FORMED (PIN) protein family of auxin transporters mediates polar auxin transport and has crucial roles in plant growth and development. Here we present cryo-electron microscopy structures of PIN3 from Arabidopsis thaliana in the apo state and in complex with its substrate indole-3-acetic acid and the inhibitor N-1-naphthylphthalamic acid (NPA). A. thaliana PIN3 exists as a homodimer, and its transmembrane helices 1, 2 and 7 in the scaffold domain are involved in dimerization. The dimeric PIN3 forms a large, joint extracellular-facing cavity at the dimer interface while each subunit adopts an inward-facing conformation. The structural and functional analyses, along with computational studies, reveal the structural basis for the recognition of indole-3-acetic acid and NPA and elucidate the molecular mechanism of NPA inhibition on PIN-mediated auxin transport. The PIN3 structures support an elevator-like model for the transport of auxin, whereby the transport domains undergo up-down rigid-body motions and the dimerized scaffold domains remain static.
External linksNature / PubMed:35917926
MethodsEM (single particle)
Resolution2.62 - 3.0 Å
Structure data

32568

7wks

EMDB-32568, PDB-7wks:
Apo state of AtPIN3
Method: EM (single particle) / Resolution: 3.0 Å

32570

7wkw

EMDB-32570, PDB-7wkw:
NPA bound state of AtPIN3
Method: EM (single particle) / Resolution: 2.62 Å

33500

7xxb

EMDB-33500, PDB-7xxb:
IAA bound state of AtPIN3
Method: EM (single particle) / Resolution: 2.93 Å

Chemicals

ChemComp-E7O:
2-(naphthalen-1-ylcarbamoyl)benzoic acid

ChemComp-IAC:
1H-INDOL-3-YLACETIC ACID / hormone*YM / Indole-3-acetic acid

Source
  • arabidopsis thaliana (thale cress)
KeywordsTRANSPORT PROTEIN / PIN-FORMED (PIN) protein / NPA

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