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TitleStructure of cyanobacterial photosystem I complexed with ferredoxin at 1.97 Å resolution.
Journal, issue, pagesCommun Biol, Vol. 5, Issue 1, Page 951, Year 2022
Publish dateSep 12, 2022
AuthorsJiannan Li / Noriyuki Hamaoka / Fumiaki Makino / Akihiro Kawamoto / Yuxi Lin / Matthias Rögner / Marc M Nowaczyk / Young-Ho Lee / Keiichi Namba / Christoph Gerle / Genji Kurisu /
PubMed AbstractPhotosystem I (PSI) is a light driven electron pump transferring electrons from Cytochrome c (Cyt c) to Ferredoxin (Fd). An understanding of this electron transfer process is hampered by a paucity of ...Photosystem I (PSI) is a light driven electron pump transferring electrons from Cytochrome c (Cyt c) to Ferredoxin (Fd). An understanding of this electron transfer process is hampered by a paucity of structural detail concerning PSI:Fd interface and the possible binding sites of Cyt c. Here we describe the high resolution cryo-EM structure of Thermosynechococcus elongatus BP-1 PSI in complex with Fd and a loosely bound Cyt c. Side chain interactions at the PSI:Fd interface including bridging water molecules are visualized in detail. The structure explains the properties of mutants of PsaE and PsaC that affect kinetics of Fd binding and suggests a molecular switch for the dissociation of Fd upon reduction. Calorimetry-based thermodynamic analyses confirms a single binding site for Fd and demonstrates that PSI:Fd complexation is purely driven by entropy. A possible reaction cycle for the efficient transfer of electrons from Cyt c to Fd via PSI is proposed.
External linksCommun Biol / PubMed:36097054 / PubMed Central
MethodsEM (single particle)
Resolution1.97 Å
Structure data

31605

7fix

EMDB-31605, PDB-7fix:
Cryo-EM structure of cyanobacterial photosystem I in the presence of ferredoxin and cytochrome c6
Method: EM (single particle) / Resolution: 1.97 Å

Chemicals

ChemComp-CL0:
CHLOROPHYLL A ISOMER / Chlorophyll a

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol


ChemComp, No image

ChemComp-UNL:
Unknown ligand

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-CA:
Unknown entry

ChemComp-GAK:
[2Ga-2S] cluster

ChemComp-HOH:
WATER / Water

Source
  • Thermosynechococcus elongatus BP-1 (bacteria)
  • thermosynechococcus vestitus bp-1 (bacteria)
KeywordsPHOTOSYNTHESIS / Photosystem I / Ferredoxin / Cytochrome c6

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