EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural and functional insights into Escherichia coli α2-macroglobulin endopeptidase snap-trap inhibition.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 112, Issue 27, Page 8290-8295, Year 2015
掲載日	2015年7月7日
著者	Irene Garcia-Ferrer / Pedro Arêde / Josué Gómez-Blanco / Daniel Luque / Stephane Duquerroy / José R Castón / Theodoros Goulas / F Xavier Gomis-Rüth /
PubMed 要旨	The survival of commensal bacteria requires them to evade host peptidases. Gram-negative bacteria from the human gut microbiome encode a relative of the human endopeptidase inhibitor, α2- ...The survival of commensal bacteria requires them to evade host peptidases. Gram-negative bacteria from the human gut microbiome encode a relative of the human endopeptidase inhibitor, α2-macroglobulin (α2M). Escherichia coli α2M (ECAM) is a ∼ 180-kDa multidomain membrane-anchored pan-peptidase inhibitor, which is cleaved by host endopeptidases in an accessible bait region. Structural studies by electron microscopy and crystallography reveal that this cleavage causes major structural rearrangement of more than half the 13-domain structure from a native to a compact induced form. It also exposes a reactive thioester bond, which covalently traps the peptidase. Subsequently, peptidase-laden ECAM is shed from the membrane and may dimerize. Trapped peptidases are still active except against very large substrates, so inhibition potentially prevents damage of large cell envelope components, but not host digestion. Mechanistically, these results document a novel monomeric "snap trap."
リンク	Proc Natl Acad Sci U S A / PubMed:26100869 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.6 - 17.0 Å
構造データ	3016 5a42 EMDB-3016, PDB-5a42: Cryo-EM single particle 3D reconstruction of the native conformation of E. coli alpha-2-macroglobulin (ECAM) 手法: EM (単粒子) / 解像度: 16.0 Å EMDB-3017: Cryo-EM single particle 3D reconstruction of the trypsin-induced dimeric conformation of E. coli alpha-2-macroglobulin (ECAM) 手法: EM (単粒子) / 解像度: 14.5 Å EMDB-3018: Cryo-EM single particle 3D reconstruction of the induced, monomeric conformation of E. coli alpha-2-macroglobulin (ECAM) 手法: EM (単粒子) / 解像度: 17.0 Å PDB-4ziq: Crystal structure of trypsin activated alpha-2-macroglobulin from Escherichia coli. 手法: X-RAY DIFFRACTION / 解像度: 2.55 Å PDB-4ziu: Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning the residues from domain MG7 to the C-terminus. 手法: X-RAY DIFFRACTION / 解像度: 2.7 Å PDB-4zjg: Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning domains MG0-NIE-MG1. 手法: X-RAY DIFFRACTION / 解像度: 2.3 Å PDB-4zjh: Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning domains NIE-MG1. 手法: X-RAY DIFFRACTION / 解像度: 1.6 Å
化合物	ChemComp-GOL: GLYCEROL / グリセロ-ル / グリセリン ChemComp-CL: Unknown entry / クロリド / 塩化物 ChemComp-HOH: WATER / 水 ChemComp-NI: NICKEL (II) ION / ニッケル ChemComp-PG4: TETRAETHYLENE GLYCOL / テトラエチレングリコ-ル / 沈殿剤YM / ポリエチレングリコール ChemComp-PGE: TRIETHYLENE GLYCOL / トリエチレングリコ-ル / ポリエチレングリコール ChemComp-1PE: PENTAETHYLENE GLYCOL / ペンタエチレングリコ-ル / 沈殿剤YM / ポリエチレングリコール ChemComp-PEG: DI(HYDROXYETHYL)ETHER / ジエチレングリコ-ル / ジエチレングリコール ChemComp-ACT: ACETATE ION / 酢酸イオン / 酢酸塩
由来	escherichia coli k-12 (大腸菌) escherichia coli (strain k12) (大腸菌)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Bacterial pan-proteinase inhibitor / MEMBRANE PROTEIN/INHIBITOR (生体膜) / membrane protein and inhibitor complex (生体膜) / MEMBRANE PROTEIN-INHIBITOR complex (生体膜) / HYDROLASE INHIBITOR / PEPTIDASE INHIBITOR (プロテアーゼ阻害剤 (生物学))