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TitleCryoEM structure of the tegumented capsid of Epstein-Barr virus.
Journal, issue, pagesCell Res, Vol. 30, Issue 10, Page 873-884, Year 2020
Publish dateJul 3, 2020
AuthorsZhihai Li / Xiao Zhang / Lili Dong / Jingjing Pang / Miao Xu / Qian Zhong / Mu-Sheng Zeng / Xuekui Yu /
PubMed AbstractEpstein-Barr virus (EBV) is the primary cause of infectious mononucleosis and has been shown to be closely associated with various malignancies. Here, we present a complete atomic model of EBV, ...Epstein-Barr virus (EBV) is the primary cause of infectious mononucleosis and has been shown to be closely associated with various malignancies. Here, we present a complete atomic model of EBV, including the icosahedral capsid, the dodecameric portal and the capsid-associated tegument complex (CATC). Our in situ portal from the tegumented capsid adopts a closed conformation with its channel valve holding the terminal viral DNA and with its crown region firmly engaged by three layers of ring-like dsDNA, which, together with the penton flexibility, effectively alleviates the capsid inner pressure placed on the portal cap. In contrast, the CATCs, through binding to the flexible penton vertices in a stoichiometric manner, accurately increase the inner capsid pressure to facilitate the pressure-driven genome delivery. Together, our results provide important insights into the mechanism by which the EBV capsid, portal, packaged genome and the CATCs coordinately achieve a pressure balance to simultaneously benefit both viral genome retention and ejection.
External linksCell Res / PubMed:32620850 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 5.5 Å
Structure data

EMDB-30146:
Epstein-Barr Virus, C1 tegumented capsid reconstruction
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-30152:
Epstein-Barr virus (EBV), C5 penton vertex
Method: EM (single particle) / Resolution: 3.5 Å

30155

7bqt

EMDB-30155, PDB-7bqt:
Epstein-Barr virus, C12 portal dodecamer
Method: EM (single particle) / Resolution: 4.8 Å

EMDB-30156:
Epstein-Barr virus, C1 portal vertex reconstruction
Method: EM (single particle) / Resolution: 5.5 Å

30157

7bqx

EMDB-30157, PDB-7bqx:
Epstein-Barr virus, C5 portal vertex
Method: EM (single particle) / Resolution: 4.2 Å

30158

7br7

EMDB-30158, PDB-7br7:
Epstein-Barr virus, C1 portal-proximal penton vertex, CATC binding
Method: EM (single particle) / Resolution: 4.3 Å

30159

7br8

EMDB-30159, PDB-7br8:
Epstein-Barr virus, C5 penton vertex, CATC absent.
Method: EM (single particle) / Resolution: 3.8 Å

30162

7bsi

EMDB-30162: Epstein-Barr virus, icosahedral tegumented capsid reconstruction
PDB-7bsi: Epstein-Barr virus, one asymmetric unit structure of the icosahedral tegumented capsid
Method: EM (single particle) / Resolution: 4.1 Å

Source
  • epstein-barr virus (strain b95-8) (Epstein-Barr virus (strain B95-8))
  • HHV-4 (Epstein-Barr virus)
KeywordsVIRAL PROTEIN / Epstein-Barr virus / portal dodecomer / tegumented capsid / portal vertex / CATC / portal-proximal penton vertex / CATC binding / asymmetric reconstruction / C5 penton vertex / CATC absent / VIRUS / icosahedral reconstruction

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