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TitleMolecular insights into the regulation of constitutive activity by RNA editing of 5HT serotonin receptors.
Journal, issue, pagesCell Rep, Vol. 40, Issue 7, Page 111211, Year 2022
Publish dateAug 16, 2022
AuthorsRyan H Gumpper / Jonathan F Fay / Bryan L Roth /
PubMed AbstractRNA editing is a process by which post-transcriptional changes of mRNA nucleotides alter protein function through modification of the amino acid content. The 5HT serotonin receptor, which undergoes ...RNA editing is a process by which post-transcriptional changes of mRNA nucleotides alter protein function through modification of the amino acid content. The 5HT serotonin receptor, which undergoes 32 distinct RNA-editing events leading to 24 protein isoforms, is a notable example of this process. These 5HT isoforms display differences in constitutive activity, agonist/inverse agonist potencies, and efficacies. To elucidate the molecular mechanisms responsible for these effects of RNA editing, we present four active-state 5HT-transducer-coupled structures of three representative isoforms (INI, VGV, and VSV) with the selective drug lorcaserin (Belviq) and the classic psychedelic psilocin. We also provide a comprehensive analysis of agonist activation and constitutive activity across all 24 protein isoforms. Collectively, these findings reveal a unique hydrogen-bonding network located on intracellular loop 2 that is subject to RNA editing, which differentially affects GPCR constitutive and agonist signaling activities.
External linksCell Rep / PubMed:35977511 / PubMed Central
MethodsEM (single particle)
Resolution2.84 - 3.6 Å
Structure data

27633

8dpf

EMDB-27633, PDB-8dpf:
Cryo-EM structure of the 5HT2C receptor (INI isoform) bound to lorcaserin
Method: EM (single particle) / Resolution: 2.84 Å

27634

8dpg

EMDB-27634, PDB-8dpg:
Cryo-EM structure of the 5HT2C receptor (INI isoform) bound to psilocin
Method: EM (single particle) / Resolution: 3.6 Å

27635

8dph

EMDB-27635, PDB-8dph:
Cryo-EM structure of the 5HT2C receptor (VGV isoform) bound to lorcaserin
Method: EM (single particle) / Resolution: 3.2 Å

27636

8dpi

EMDB-27636, PDB-8dpi:
Cryo-EM structure of the 5HT2C receptor (VSV isoform) bound to lorcaserin
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-T4U:
(1R)-8-chloro-1-methyl-2,3,4,5-tetrahydro-1H-3-benzazepine / Lorcaserin

ChemComp-CLR:
CHOLESTEROL / Cholesterol

ChemComp-91Q:
3-[2-(dimethylamino)ethyl]-1~{H}-indol-4-ol / alkaloid*YM / Psilocin

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / GPCR

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