EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-ET of parasites gives subnanometer insight into tubulin-based structures.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 119, Issue 6, Year 2022
掲載日	2022年2月8日
著者	Stella Y Sun / Li-Av Segev-Zarko / Muyuan Chen / Grigore D Pintilie / Michael F Schmid / Steven J Ludtke / John C Boothroyd / Wah Chiu /
PubMed 要旨	Tubulin is a conserved protein that polymerizes into different forms of filamentous structures in , an obligate intracellular parasite in the phylum Apicomplexa. Two key tubulin-containing ...Tubulin is a conserved protein that polymerizes into different forms of filamentous structures in , an obligate intracellular parasite in the phylum Apicomplexa. Two key tubulin-containing cytoskeletal components are subpellicular microtubules (SPMTs) and conoid fibrils (CFs). The SPMTs help maintain shape and gliding motility, while the CFs are implicated in invasion. Here, we use cryogenic electron tomography to determine the molecular structures of the SPMTs and CFs in vitrified intact and detergent-extracted parasites. Subvolume densities from detergent-extracted parasites yielded averaged density maps at subnanometer resolutions, and these were related back to their architecture in situ. An intralumenal spiral lines the interior of the 13-protofilament SPMTs, revealing a preferred orientation of these microtubules relative to the parasite's long axis. Each CF is composed of nine tubulin protofilaments that display a comma-shaped cross-section, plus additional associated components. Conoid protrusion, a crucial step in invasion, is associated with an altered pitch of each CF. The use of basic building blocks of protofilaments and different accessory proteins in one organism illustrates the versatility of tubulin to form two distinct types of assemblies, SPMTs and CFs.
リンク	Proc Natl Acad Sci U S A / PubMed:35121661 / PubMed Central
手法	EM (トモグラフィー) / EM (サブトモグラム平均)
解像度	6.7 - 46.0 Å
構造データ	EMDB-26006: 3D reconstruction of detergent-extract Toxoplasma gondii cells at the apical end 手法: EM (トモグラフィー) EMDB-26007: Three-dimensional organization of the apical complex in Toxoplasma tachyzoites 手法: EM (トモグラフィー) EMDB-26008: In situ average map of conoid with PCR from intact Toxoplasma gondii cells 手法: EM (サブトモグラム平均) / 解像度: 46.0 Å EMDB-26009: The conoid segment from intact Toxoplasma gondii cells 手法: EM (サブトモグラム平均) / 解像度: 28.0 Å EMDB-26010: The symmetrized subpellicular microtubule map from intact Toxoplasma gondii cells 手法: EM (サブトモグラム平均) / 解像度: 26.0 Å 26018 7tnq EMDB-26018, PDB-7tnq: The symmetry-released subpellicular microtubule map from detergent-extracted Toxoplasma cells 手法: EM (サブトモグラム平均) / 解像度: 8.4 Å 26019 7tns EMDB-26019, PDB-7tns: Subpellicular microtubule from detergent-extract Toxoplasma gondii cells 手法: EM (サブトモグラム平均) / 解像度: 6.7 Å 26020 7tnt EMDB-26020, PDB-7tnt: The tubulin-based conoid from detergent-extract Toxoplasma gondii cells 手法: EM (サブトモグラム平均) / 解像度: 9.3 Å
由来	toxoplasma gondii (トキソプラズマ)
キーワード	CELL INVASION / parasites (寄生) / Toxoplasma gondii (トキソプラズマ) / cytoskeleton (細胞骨格) / microtubules (微小管) / tubulin (チューブリン) / conoid