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TitleCharacterization of prefusion-F-specific antibodies elicited by natural infection with human metapneumovirus.
Journal, issue, pagesCell Rep, Vol. 40, Issue 12, Page 111399, Year 2022
Publish dateSep 20, 2022
AuthorsScott A Rush / Gurpreet Brar / Ching-Lin Hsieh / Emilie Chautard / Jennifer N Rainho-Tomko / Chris D Slade / Christine A Bricault / Ana Kume / James Kearns / Rachel Groppo / Sophia T Mundle / Linong Zhang / Danilo Casimiro / Tong-Ming Fu / Joshua M DiNapoli / Jason S McLellan /
PubMed AbstractHuman metapneumovirus (hMPV) is a major cause of acute respiratory infections in infants and older adults, for which no vaccines or therapeutics are available. The viral fusion (F) glycoprotein is ...Human metapneumovirus (hMPV) is a major cause of acute respiratory infections in infants and older adults, for which no vaccines or therapeutics are available. The viral fusion (F) glycoprotein is required for entry and is the primary target of neutralizing antibodies; however, little is known about the humoral immune response generated from natural infection. Here, using prefusion-stabilized F proteins to interrogate memory B cells from two older adults, we obtain over 700 paired non-IgM antibody sequences representing 563 clonotypes, indicative of a highly polyclonal response. Characterization of 136 monoclonal antibodies reveals broad recognition of the protein surface, with potently neutralizing antibodies targeting each antigenic site. Cryo-EM studies further reveal two non-canonical sites and the molecular basis for recognition of the apex of hMPV F by two prefusion-specific neutralizing antibodies. Collectively, these results provide insight into the humoral response to hMPV infection in older adults and will help guide vaccine development.
External linksCell Rep / PubMed:36130517
MethodsEM (single particle)
Resolution3.06 - 3.13 Å
Structure data

25929

7tjq

EMDB-25929, PDB-7tjq:
SAN27-14 bound to a antigenic site V on prefusion-stabilized hMPV F
Method: EM (single particle) / Resolution: 3.13 Å

25982

7tl0

EMDB-25982, PDB-7tl0:
Cryo-EM structure of hMPV preF bound by Fabs MPE8 and SAN32-2
Method: EM (single particle) / Resolution: 3.06 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • homo sapiens (human)
  • human metapneumovirus
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / neutralizing antibodies / prefusion-specific antibodies / antigenic site V / hMPV F / VIRAL PROTEIN-IMMUNE SYSTEM complex / neutralizing antibody / fusion protein / metapneumovirus / site 0

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