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TitleStructure of the mammalian oligosaccharyl-transferase complex in the native ER protein translocon.
Journal, issue, pagesNat Commun, Vol. 5, Page 3072, Year 2014
Publish dateNov 24, 2015
AuthorsStefan Pfeffer / Johanna Dudek / Marko Gogala / Stefan Schorr / Johannes Linxweiler / Sven Lang / Thomas Becker / Roland Beckmann / Richard Zimmermann / Friedrich Förster /
PubMed AbstractIn mammalian cells, proteins are typically translocated across the endoplasmic reticulum (ER) membrane in a co-translational mode by the ER protein translocon, comprising the protein-conducting ...In mammalian cells, proteins are typically translocated across the endoplasmic reticulum (ER) membrane in a co-translational mode by the ER protein translocon, comprising the protein-conducting channel Sec61 and additional complexes involved in nascent chain processing and translocation. As an integral component of the translocon, the oligosaccharyl-transferase complex (OST) catalyses co-translational N-glycosylation, one of the most common protein modifications in eukaryotic cells. Here we use cryoelectron tomography, cryoelectron microscopy single-particle analysis and small interfering RNA-mediated gene silencing to determine the overall structure, oligomeric state and position of OST in the native ER protein translocon of mammalian cells in unprecedented detail. The observed positioning of OST in close proximity to Sec61 provides a basis for understanding how protein translocation into the ER and glycosylation of nascent proteins are structurally coupled. The overall spatial organization of the native translocon, as determined here, serves as a reliable framework for further hypothesis-driven studies.
External linksNat Commun / PubMed:24407213
MethodsEM (subtomogram averaging) / EM (single particle)
Resolution9.3 - 40.0 Å
Structure data

EMDB-2514:
ER membrane-associated ribosome from HeLa cells after treatment with non-silencing control siRNA
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

EMDB-2515:
ER membrane-associated ribosome from HeLa cells after treatment with TRAP-beta siRNA
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

EMDB-2516:
ER membrane-associated ribosome from HeLa cells after treatment with Ribophorin I siRNA
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

EMDB-2517:
ER membrane-associated ribosome from HeLa cells after treatment with Ribophorin II siRNA
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

EMDB-2518:
ER membrane-associated ribosome from HeLa cells after treatment with SPC25 siRNA
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

EMDB-2519:
Stalled mammalian ribosome bound to canine pancreatic microsomes
Method: EM (subtomogram averaging) / Resolution: 22.0 Å

EMDB-2523:
Structure of the mammalian oligosaccharyl-transferase complex in the native ER protein translocon
Method: EM (single particle) / Resolution: 9.3 Å

Source
  • Homo sapiens (human)
  • Oryctolagus cuniculus (rabbit)
  • Canis lupus familiaris (dog)
  • Triticum aestivum (bread wheat)

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