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TitleCryo-EM of CcsBA reveals the basis for cytochrome c biogenesis and heme transport.
Journal, issue, pagesNat Chem Biol, Vol. 18, Issue 1, Page 101-108, Year 2022
Publish dateDec 20, 2021
AuthorsDeanna L Mendez / Ethan P Lowder / Dustin E Tillman / Molly C Sutherland / Andrea L Collier / Michael J Rau / James A J Fitzpatrick / Robert G Kranz /
PubMed AbstractAlthough the individual structures and respiratory functions of cytochromes are well studied, the structural basis for their assembly, including transport of heme for attachment, are unknown. We ...Although the individual structures and respiratory functions of cytochromes are well studied, the structural basis for their assembly, including transport of heme for attachment, are unknown. We describe cryo-electron microscopy (cryo-EM) structures of CcsBA, a bifunctional heme transporter and cytochrome c (cyt c) synthase. Models built from the cryo-EM densities show that CcsBA is trapped with heme in two conformations, herein termed the closed and open states. The closed state has heme located solely at a transmembrane (TM) site, with a large periplasmic domain oriented such that access of heme to the cytochrome acceptor is denied. The open conformation contains two heme moieties, one in the TM-heme site and another in an external site (P-heme site). The presence of heme in the periplasmic site at the base of a chamber induces a large conformational shift that exposes the heme for reaction with apocytochrome c (apocyt c). Consistent with these structures, in vivo and in vitro cyt c synthase studies suggest a mechanism for transfer of the periplasmic heme to cytochrome.
External linksNat Chem Biol / PubMed:34931065 / PubMed Central
MethodsEM (single particle)
Resolution3.56 - 4.14 Å
Structure data

24941

7s9y

EMDB-24941, PDB-7s9y:
Helicobacter Hepaticus CcsBA Open Conformation
Method: EM (single particle) / Resolution: 3.56 Å

24942

7s9z

EMDB-24942, PDB-7s9z:
Helicobacter Hepaticus CcsBA Closed Conformation
Method: EM (single particle) / Resolution: 4.14 Å

Chemicals

ChemComp-HEB:
HEME B/C

ChemComp-PTY:
PHOSPHATIDYLETHANOLAMINE / phospholipid*YM / Phosphatidylethanolamine

Source
  • helicobacter hepaticus (bacteria)
KeywordsMEMBRANE PROTEIN / Cytochrome c biogenesis / Heme transporter / Heme lyase

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