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TitleStructure of a type IV secretion system core complex encoded by multi-drug resistance F plasmids.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 379, Year 2022
Publish dateJan 19, 2022
AuthorsXiangan Liu / Pratick Khara / Matthew L Baker / Peter J Christie / Bo Hu /
PubMed AbstractBacterial type IV secretion systems (T4SSs) are largely responsible for the proliferation of multi-drug resistance. We solved the structure of the outer-membrane core complex (OMCC) of a T4SS encoded ...Bacterial type IV secretion systems (T4SSs) are largely responsible for the proliferation of multi-drug resistance. We solved the structure of the outer-membrane core complex (OMCC) of a T4SS encoded by a conjugative F plasmid at <3.0 Å resolution by cryoelectron microscopy. The OMCC consists of a 13-fold symmetrical outer ring complex (ORC) built from 26 copies of TraK and TraV C-terminal domains, and a 17-fold symmetrical central cone (CC) composed of 17 copies of TraB β-barrels. Domains of TraV and TraB also bind the CC and ORC substructures, establishing that these proteins undergo an intraprotein symmetry alteration to accommodate the C13:C17 symmetry mismatch. We present evidence that other pED208-encoded factors stabilize the C13:C17 architecture and define the importance of TraK, TraV and TraB domains to T4SS function. This work identifies OMCC structural motifs of proposed importance for structural transitions associated with F plasmid dissemination and F pilus biogenesis.
External linksNat Commun / PubMed:35046412 / PubMed Central
MethodsEM (single particle)
Resolution2.95 - 4.22 Å
Structure data

EMDB-24768:
C1 reconstruction for Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by F-plasmid (pED208)
Method: EM (single particle) / Resolution: 4.22 Å

24769

7spb

EMDB-24769: C13 reconstruction for Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by F-plasmid (pED208).
PDB-7spb: Models for C13 reconstruction of Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by F-plasmid (pED208).
Method: EM (single particle) / Resolution: 3.31 Å

24770

7spc

EMDB-24770: C17 reconstruction for Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by F-plasmid (pED208)
PDB-7spc: Models for C17 reconstruction of Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by F-plasmid (pED208).
Method: EM (single particle) / Resolution: 2.95 Å

24771

7spi

EMDB-24771: C13 reconstruction for Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by a plasmid overproducing TraV, TraK and TraB of pED208
PDB-7spi: Models for C13 reconstruction of Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by a plasmid overproducing TraV, TraK and TraB of pED208
Method: EM (single particle) / Resolution: 2.97 Å

24772

7spj

EMDB-24772: C17 reconstruction for Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by a plasmid overproducing TraV, TraK and TraB of pED208
PDB-7spj: Models for C17 reconstruction of Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by a plasmid overproducing TraV, TraK and TraB of pED208
Method: EM (single particle) / Resolution: 3.54 Å

24773

7spk

EMDB-24773: C16 reconstruction for Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by a plasmid overproducing TraV, TraK and TraB of pED208
PDB-7spk: Models for C16 reconstruction of Outer Membrane Core Complex (OMCC) of Type IV Secretion System (T4SS) encoded by a plasmid overproducing TraV, TraK and TraB of pED208
Method: EM (single particle) / Resolution: 3.87 Å

Source
  • Escherichia coli (E. coli)
  • Escherichia coli BL21(DE3) (bacteria)
  • salmonella typhi (bacteria)
KeywordsSTRUCTURAL PROTEIN / Symmetry alteration / Symmetry mismatch / Drug resistance / Type IV secretion system (T4SS)

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