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TitleStructural mechanism for gating of a eukaryotic mechanosensitive channel of small conductance.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 3690, Year 2020
Publish dateJul 23, 2020
AuthorsZengqin Deng / Grigory Maksaev / Angela M Schlegel / Jingying Zhang / Michael Rau / James A J Fitzpatrick / Elizabeth S Haswell / Peng Yuan /
PubMed AbstractMechanosensitive ion channels transduce physical force into electrochemical signaling that underlies an array of fundamental physiological processes, including hearing, touch, proprioception, ...Mechanosensitive ion channels transduce physical force into electrochemical signaling that underlies an array of fundamental physiological processes, including hearing, touch, proprioception, osmoregulation, and morphogenesis. The mechanosensitive channels of small conductance (MscS) constitute a remarkably diverse superfamily of channels critical for management of osmotic pressure. Here, we present cryo-electron microscopy structures of a MscS homolog from Arabidopsis thaliana, MSL1, presumably in both the closed and open states. The heptameric MSL1 channel contains an unusual bowl-shaped transmembrane region, which is reminiscent of the evolutionarily and architecturally unrelated mechanosensitive Piezo channels. Upon channel opening, the curved transmembrane domain of MSL1 flattens and expands. Our structures, in combination with functional analyses, delineate a structural mechanism by which mechanosensitive channels open under increased membrane tension. Further, the shared structural feature between unrelated channels suggests the possibility of a unified mechanical gating mechanism stemming from membrane deformation induced by a non-planar transmembrane domain.
External linksNat Commun / PubMed:32704140 / PubMed Central
MethodsEM (single particle)
Resolution2.96 - 3.39 Å
Structure data

21444

6vxm

EMDB-21444, PDB-6vxm:
Cryo-EM structure of Arabidopsis thaliana MSL1
Method: EM (single particle) / Resolution: 3.06 Å

21445

6vxn

EMDB-21445, PDB-6vxn:
Cryo-EM structure of Arabidopsis thaliana MSL1 A320V
Method: EM (single particle) / Resolution: 2.96 Å

21447

6vxp

EMDB-21447, PDB-6vxp:
Cryo-EM structure of Arabidopsis thaliana MSL1 in lipid nanodisc
Method: EM (single particle) / Resolution: 3.39 Å

Chemicals

ChemComp-LFA:
EICOSANE / Icosane

ChemComp-D12:
DODECANE / Dodecane

Source
  • arabidopsis thaliana (thale cress)
KeywordsTRANSPORT PROTEIN / ion channel

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