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Structure paper

TitleStructure of the multi-subunit chloroplast RNA polymerase.
Journal, issue, pagesMol Cell, Vol. 84, Issue 5, Page 910-925.e5, Year 2024
Publish dateMar 7, 2024
AuthorsPaula F V do Prado / Frederik M Ahrens / Monique Liebers / Noah Ditz / Hans-Peter Braun / Thomas Pfannschmidt / Hauke S Hillen /
PubMed AbstractChloroplasts contain a dedicated genome that encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase ...Chloroplasts contain a dedicated genome that encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. Here, we report the cryo-EM structure of a 19-subunit PEP complex from Sinapis alba (white mustard). The structure reveals that the PEP core resembles prokaryotic and nuclear RNAPs but contains chloroplast-specific features that mediate interactions with the PAPs. The PAPs are unrelated to known transcription factors and arrange around the core in a unique fashion. Their structures suggest potential functions during transcription in the chemical environment of chloroplasts. These results reveal structural insights into chloroplast transcription and provide a framework for understanding photosynthesis gene expression.
External linksMol Cell / PubMed:38428434
MethodsEM (single particle)
Resolution2.83 - 3.5 Å
Structure data

18496

8qma

EMDB-18496, PDB-8qma:
Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-18499: Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba - Map A
Method: EM (single particle) / Resolution: 3.43 Å

EMDB-18500: Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba - Map B.
Method: EM (single particle) / Resolution: 2.95 Å

EMDB-18502: Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba - Map D
Method: EM (single particle) / Resolution: 3.34 Å

EMDB-18503: Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba.- Map E
Method: EM (single particle) / Resolution: 2.89 Å

EMDB-18504: Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba - Map F
Method: EM (single particle) / Resolution: 2.83 Å

Chemicals

ChemComp-FE:
Unknown entry / Iron

ChemComp-ZN:
Unknown entry

ChemComp-SAM:
S-ADENOSYLMETHIONINE / S-Adenosyl methionine

Source
  • sinapis alba (white mustard)
KeywordsTRANSCRIPTION / Chloroplasts / Gene Expression / RNA / Polymerase

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