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TitleTriple-Stranded DNA As a Structural Element in DNA Origami.
Journal, issue, pagesACS Nano, Vol. 17, Issue 10, Page 9014-9024, Year 2023
Publish dateMay 23, 2023
AuthorsKen Sachenbacher / Ali Khoshouei / Maximilian Nicolas Honemann / Wouter Engelen / Elija Feigl / Hendrik Dietz /
PubMed AbstractMolecular self-assembly with DNA origami offers an attractive route to fabricate arbitrary three-dimensional nanostructures. In DNA origami, B-form double-helical DNA domains (dsDNA) are commonly ...Molecular self-assembly with DNA origami offers an attractive route to fabricate arbitrary three-dimensional nanostructures. In DNA origami, B-form double-helical DNA domains (dsDNA) are commonly linked with covalent phosphodiester strand crossovers to build up three-dimensional objects. To expand the palette of structural motifs in DNA origami, here we describe hybrid duplex-triplex DNA motifs as pH-dependent building blocks in DNA origami. We investigate design rules for incorporating triplex forming oligonucleotides and noncanonical duplex-triplex crossovers in multilayer DNA origami objects. We use single-particle cryoelectron microscopy to elucidate the structural basis of triplex domains and of duplex-triplex crossovers. We find that duplex-triplex crossovers can complement and fully replace the canonical duplex-duplex crossovers within DNA origami objects, for example, to increase the crossover density for potentially greater rigidity and reduced interhelical spacing, and to create connections at sites where conventional crossovers may be undesirable. We also show the pH-induced formation of a DNA origami object stabilized entirely by triplex-mediated strand crossovers.
External linksACS Nano / PubMed:37159224 / PubMed Central
MethodsEM (single particle)
Resolution7.27 - 7.31 Å
Structure data

EMDB-16213: Triple-stranded DNA as a structural element in DNA origami large cuboid object
Method: EM (single particle) / Resolution: 7.31 Å

EMDB-16220: Triple-stranded DNA as a structural element in DNA origami reinforced rod object
Method: EM (single particle) / Resolution: 7.27 Å

EMDB-16221: Triple-stranded DNA as a structural element in DNA origami rod object
Method: EM (single particle) / Resolution: 7.27 Å

Source
  • synthetic construct (others)
  • Phage M13mp18 (virus)

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