Luke Smithers / Oksana Degtjarik / Dietmar Weichert / Chia-Ying Huang / Coilín Boland / Katherine Bowen / Abraham Oluwole / Corinne Lutomski / Carol V Robinson / Eoin M Scanlan / Meitian Wang / Vincent Olieric / Moran Shalev-Benami / Martin Caffrey /
PubMed Abstract
Bacterial lipoproteins (BLPs) decorate the surface of membranes in the cell envelope. They function in membrane assembly and stability, as enzymes, and in transport. The final enzyme in the BLP ...Bacterial lipoproteins (BLPs) decorate the surface of membranes in the cell envelope. They function in membrane assembly and stability, as enzymes, and in transport. The final enzyme in the BLP synthesis pathway is the apolipoprotein -acyltransferase, Lnt, which is proposed to act by a ping-pong mechanism. Here, we use x-ray crystallography and cryo-electron microscopy to chart the structural changes undergone during the progress of the enzyme through the reaction. We identify a single active site that has evolved to bind, individually and sequentially, substrates that satisfy structural and chemical criteria to position reactive parts next to the catalytic triad for reaction. This study validates the ping-pong mechanism, explains the molecular bases for Lnt's substrate promiscuity, and should facilitate the design of antibiotics with minimal off-target effects.
EMDB-15786, PDB-8b0k: Cryo-EM structure of apolipoprotein N-acyltransferase Lnt from E. coli (Apo form) Method: EM (single particle) / Resolution: 3.0 Å
EMDB-15787, PDB-8b0l: Cryo-EM structure of apolipoprotein N-acyltransferase Lnt from E. coli in complex with PE Method: EM (single particle) / Resolution: 3.13 Å
EMDB-15788, PDB-8b0m: Cryo-EM structure of apolipoprotein N-acyltransferase Lnt from E. coli in complex with PE (C387S mutant) Method: EM (single particle) / Resolution: 3.01 Å
EMDB-15789, PDB-8b0n: Cryo-EM structure of apolipoprotein N-acyltransferase Lnt from E. coli in complex with Lyso-PE Method: EM (single particle) / Resolution: 2.67 Å
EMDB-15790, PDB-8b0o: Cryo-EM structure apolipoprotein N-acyltransferase Lnt from E.coli in complex with FP3 Method: EM (single particle) / Resolution: 3.02 Å
EMDB-15791, PDB-8b0p: Cryo-EM structure of apolipoprotein N-acyltransferase Lnt from E. coli in complex with Pam3 Method: EM (single particle) / Resolution: 2.86 Å
PDB-8aq2: In meso structure of the membrane integral lipoprotein N-acyltransferase Lnt from P. aeruginosa covalently linked with TITC Method: X-RAY DIFFRACTION / Resolution: 2.6 Å
PDB-8aq3: In surfo structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli in complex with PE Method: X-RAY DIFFRACTION / Resolution: 2.395 Å
PDB-8aq4: In surfo structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli in complex with TITC and lyso-PE Method: X-RAY DIFFRACTION / Resolution: 2.62 Å
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Keywords
TRANSFERASE / Lnt / apolipoprotein N-acyltransferase / Bacterial lipoproteins. / 
