EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of bacteriophage T5 infection trigger and cell wall perforation.
ジャーナル・号・ページ	Sci Adv, Vol. 9, Issue 12, Page eade9674, Year 2023
掲載日	2023年3月24日
著者	Romain Linares / Charles-Adrien Arnaud / Grégory Effantin / Claudine Darnault / Nathan Hugo Epalle / Elisabetta Boeri Erba / Guy Schoehn / Cécile Breyton /
PubMed 要旨	Most bacteriophages present a tail allowing host recognition, cell wall perforation, and viral DNA channeling from the capsid to the infected bacterium cytoplasm. The majority of tailed phages bear a ...Most bacteriophages present a tail allowing host recognition, cell wall perforation, and viral DNA channeling from the capsid to the infected bacterium cytoplasm. The majority of tailed phages bear a long flexible tail () at the tip of which receptor binding proteins (RBPs) specifically interact with their host, triggering infection. In siphophage T5, the unique RBP is located at the extremity of a central fiber. We present the structures of T5 tail tip, determined by cryo-electron microscopy before and after interaction with its receptor, FhuA, reconstituted into nanodisc. These structures bring out the important conformational changes undergone by T5 tail tip upon infection, which include bending of T5 central fiber on the side of the tail tip, tail anchoring to the membrane, tail tube opening, and formation of a transmembrane channel. The data allow to detail the first steps of an otherwise undescribed infection mechanism.
リンク	Sci Adv / PubMed:36961893 / PubMed Central
手法	EM (単粒子)
解像度	3.45 - 4.32 Å
構造データ	13953 7qg9 EMDB-13953, PDB-7qg9: Tail tip of siphophage T5 : common core proteins 手法: EM (単粒子) / 解像度: 3.45 Å 14733 7zhj EMDB-14733, PDB-7zhj: Tail tip of siphophage T5 : tip proteins 手法: EM (単粒子) / 解像度: 3.53 Å 14790 7zlv EMDB-14790: Tail tip of siphophage T5 : central fibre PDB-7zlv: Tail tip of siphophage T5 : central fibre protein pb4 手法: EM (単粒子) / 解像度: 4.22 Å 14799 7zn2 EMDB-14799, PDB-7zn2: Tail tip of siphophage T5 : full complex after interaction with its bacterial receptor FhuA 手法: EM (単粒子) / 解像度: 4.29 Å 14800 7zn4 EMDB-14800, PDB-7zn4: Tail tip of siphophage T5 : bent fibre after interaction with its bacterial receptor FhuA 手法: EM (単粒子) / 解像度: 4.32 Å 14869 7zqb EMDB-14869, PDB-7zqb: Tail tip of siphophage T5 : full structure 手法: EM (単粒子) / 解像度: 3.88 Å 14873 7zqp EMDB-14873, PDB-7zqp: Tail tip of siphophage T5 : open cone after interaction with bacterial receptor FhuA 手法: EM (単粒子) / 解像度: 3.6 Å
由来	Enterobacteria phage T5 (ファージ) escherichia phage t5 (ファージ)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / Bacteriophage (ファージ) / Siphophage / T5 / baseplate (三脚) / VIRUS LIKE PARTICLE (ウイルス様粒子)