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TitleAllosteric modulation of LRRC8 channels by targeting their cytoplasmic domains.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 5435, Year 2021
Publish dateSep 14, 2021
AuthorsDawid Deneka / Sonja Rutz / Cedric A J Hutter / Markus A Seeger / Marta Sawicka / Raimund Dutzler /
PubMed AbstractMembers of the LRRC8 family form heteromeric assemblies, which function as volume-regulated anion channels. These modular proteins consist of a transmembrane pore and cytoplasmic leucine-rich repeat ...Members of the LRRC8 family form heteromeric assemblies, which function as volume-regulated anion channels. These modular proteins consist of a transmembrane pore and cytoplasmic leucine-rich repeat (LRR) domains. Despite their known molecular architecture, the mechanism of activation and the role of the LRR domains in this process has remained elusive. Here we address this question by generating synthetic nanobodies, termed sybodies, which target the LRR domain of the obligatory subunit LRRC8A. We use these binders to investigate their interaction with homomeric LRRC8A channels by cryo-electron microscopy and the consequent effect on channel activation by electrophysiology. The five identified sybodies either inhibit or enhance activity by binding to distinct epitopes of the LRR domain, thereby altering channel conformations. In combination, our work provides a set of specific modulators of LRRC8 proteins and reveals the role of their cytoplasmic domains as regulators of channel activity by allosteric mechanisms.
External linksNat Commun / PubMed:34521847 / PubMed Central
MethodsEM (single particle)
Resolution3.06 - 7.67 Å
Structure data

13202

7p5v

EMDB-13202: Cryo-EM structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1
PDB-7p5v: Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1
Method: EM (single particle) / Resolution: 3.06 Å

13203

7p5w

EMDB-13203: Cryo-EM structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb2
PDB-7p5w: Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb2
Method: EM (single particle) / Resolution: 3.5 Å

13208

7p5y

EMDB-13208, PDB-7p5y:
Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb3
Method: EM (single particle) / Resolution: 3.29 Å

EMDB-13212:
Cryo-EM structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb4 at 1:1 ratio
Method: EM (single particle) / Resolution: 7.67 Å

13213

7p60

EMDB-13213, PDB-7p60:
Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb4 at 1:0.5 ratio
Method: EM (single particle) / Resolution: 3.8 Å

13230

7p6k

EMDB-13230: Cryo-EM structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb5
PDB-7p6k: Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb5
Method: EM (single particle) / Resolution: 3.8 Å

Source
  • mus musculus (house mouse)
  • Synthetic construct (others)
KeywordsMEMBRANE PROTEIN / LRRC8 family / Volume-Regulated Anion Channel / leucine-rich repeat / sybody / cryo-EM

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