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TitleAn expanded conformation of single-ring GroEL-GroES complex encapsulates an 86 kDa substrate.
Journal, issue, pagesStructure, Vol. 14, Issue 11, Page 1711-1722, Year 2006
Publish dateJan 16, 2007
AuthorsDong-Hua Chen / Jiu-Li Song / David T Chuang / Wah Chiu / Steven J Ludtke /
PubMed AbstractElectron cryomicroscopy reveals an unprecedented conformation of the single-ring mutant of GroEL (SR398) bound to GroES in the presence of Mg-ATP. This conformation exhibits a considerable expansion ...Electron cryomicroscopy reveals an unprecedented conformation of the single-ring mutant of GroEL (SR398) bound to GroES in the presence of Mg-ATP. This conformation exhibits a considerable expansion of the folding cavity, with approximately 80% more volume than the X-ray structure of the equivalent cis cavity in the GroEL-GroES-(ADP)(7) complex. This expanded conformation can encapsulate an 86 kDa heterodimeric (alphabeta) assembly intermediate of mitochondrial branched-chain alpha-ketoacid dehydrogenase, the largest substrate ever observed to be cis encapsulated. The SR398-GroES-Mg-ATP complex is found to exist as a mixture of standard and expanded conformations, regardless of the absence or presence of the substrate. However, the presence of even a small substrate causes a pronounced bias toward the expanded conformation. Encapsulation of the large assembly intermediate is supported by a series of electron cryomicroscopy studies as well as the protection of both alpha and beta subunits of the substrate from tryptic digestion.
External linksStructure / PubMed:17098196
MethodsEM (single particle)
Resolution20.0 Å
Structure data

EMDB-1286:
An expanded conformation of single-ring GroEL-GroES complex encapsulates an 86 kDa substrate.
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-1289:
An expanded conformation of single-ring GroEL-GroES complex encapsulates an 86 kDa substrate.
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-1295:
An expanded conformation of single-ring GroEL-GroES complex encapsulates an 86 kDa substrate.
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-1296:
An expanded conformation of single-ring GroEL-GroES complex encapsulates an 86 kDa substrate.
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-1297:
An expanded conformation of single-ring GroEL-GroES complex encapsulates an 86 kDa substrate.
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-1298:
An expanded conformation of single-ring GroEL-GroES complex encapsulates an 86 kDa substrate.
Method: EM (single particle) / Resolution: 20.0 Å

Source
  • Escherichia coli (E. coli)

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