Movie
Controller
Structure viewers
About Yorodumi Papers
+Search query
-Structure paper
| Title | Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX. |
|---|---|
| Journal, issue, pages | J Virol, Vol. 80, Issue 23, Page 11881-11886, Year 2006 |
| Publish date | Sep 20, 2006 |
 Authors | Michael P Marsh / Samuel K Campos / Matthew L Baker / Christopher Y Chen / Wah Chiu / Michael A Barry /  |
| PubMed Abstract | Recombinant human adenovirus is a useful gene delivery vector for clinical gene therapy. Minor capsid protein IX of adenovirus has been of recent interest since multiple studies have shown that ...Recombinant human adenovirus is a useful gene delivery vector for clinical gene therapy. Minor capsid protein IX of adenovirus has been of recent interest since multiple studies have shown that modifications can be made to its C terminus to alter viral tropism or add molecular tags and/or reporter proteins. We examined the structure of an engineered adenovirus displaying the enhanced green fluorescent protein (EGFP) fused to the C terminus of protein IX. Cryoelectron microscopy and reconstruction localized the C-terminal EGFP fusion between the H2 hexon and the H4 hexon, positioned between adjacent facets, directly above the density previously assigned as protein IIIa. The original assignment of IIIa was based largely on indirect evidence, and the data presented herein support the reassignment of the IIIa density as protein IX. |
 External links | J Virol / PubMed:16987967 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 22.0 Å |
| Structure data |  EMDB-1272: |
