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TitleDynamic closed states of a ligand-gated ion channel captured by cryo-EM and simulations.
Journal, issue, pagesLife Sci Alliance, Vol. 4, Issue 8, Year 2021
Publish dateJul 1, 2021
AuthorsUrška Rovšnik / Yuxuan Zhuang / Björn O Forsberg / Marta Carroni / Linnea Yvonnesdotter / Rebecca J Howard / Erik Lindahl /
PubMed AbstractLigand-gated ion channels are critical mediators of electrochemical signal transduction across evolution. Biophysical and pharmacological characterization of these receptor proteins relies on high- ...Ligand-gated ion channels are critical mediators of electrochemical signal transduction across evolution. Biophysical and pharmacological characterization of these receptor proteins relies on high-quality structures in multiple, subtly distinct functional states. However, structural data in this family remain limited, particularly for resting and intermediate states on the activation pathway. Here, we report cryo-electron microscopy (cryo-EM) structures of the proton-activated ligand-gated ion channel (GLIC) under three pH conditions. Decreased pH was associated with improved resolution and side chain rearrangements at the subunit/domain interface, particularly involving functionally important residues in the β1-β2 and M2-M3 loops. Molecular dynamics simulations substantiated flexibility in the closed-channel extracellular domains relative to the transmembrane ones and supported electrostatic remodeling around E35 and E243 in proton-induced gating. Exploration of secondary cryo-EM classes further indicated a low-pH population with an expanded pore. These results allow us to define distinct protonation and activation steps in pH-stimulated conformational cycling in GLIC, including interfacial rearrangements largely conserved in the pentameric channel family.
External linksLife Sci Alliance / PubMed:34210687 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 4.1 Å
Structure data

11202

6zgd

EMDB-11202, PDB-6zgd:
GLIC pentameric ligand-gated ion channel, pH 7
Method: EM (single particle) / Resolution: 4.1 Å

11208

6zgj

EMDB-11208, PDB-6zgj:
GLIC pentameric ligand-gated ion channel, pH 5
Method: EM (single particle) / Resolution: 3.4 Å

11209

6zgk

EMDB-11209, PDB-6zgk:
GLIC pentameric ligand-gated ion channel, pH 3
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-12675:
GLIC, pentameric ligand gated ion channel, pH 3 State 2
Method: EM (single particle)

EMDB-12677:
GLIC, pentameric ligand-gated ion channel, pH 5, state 2
Method: EM (single particle)

EMDB-12678:
GLIC, pentameric ligand-gated ion channel, pH 7 state 2
Method: EM (single particle)

Source
  • gloeobacter violaceus (strain atcc 29082 / pcc 7421) (bacteria)
  • Gloeobacter violaceus (bacteria)
KeywordsMEMBRANE PROTEIN / GLIC / pentameric ligand-gated ion channel / cryoem

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