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TitleKinetic mechanism of Na-coupled aspartate transport catalyzed by Glt.
Journal, issue, pagesCommun Biol, Vol. 4, Issue 1, Page 751, Year 2021
Publish dateJun 17, 2021
AuthorsGianluca Trinco / Valentina Arkhipova / Alisa A Garaeva / Cedric A J Hutter / Markus A Seeger / Albert Guskov / Dirk J Slotboom /
PubMed AbstractIt is well-established that the secondary active transporters Glt and Glt catalyze coupled uptake of aspartate and three sodium ions, but insight in the kinetic mechanism of transport is fragmentary. ...It is well-established that the secondary active transporters Glt and Glt catalyze coupled uptake of aspartate and three sodium ions, but insight in the kinetic mechanism of transport is fragmentary. Here, we systematically measured aspartate uptake rates in proteoliposomes containing purified Glt, and derived the rate equation for a mechanism in which two sodium ions bind before and another after aspartate. Re-analysis of existing data on Glt using this equation allowed for determination of the turnover number (0.14 s), without the need for error-prone protein quantification. To overcome the complication that purified transporters may adopt right-side-out or inside-out membrane orientations upon reconstitution, thereby confounding the kinetic analysis, we employed a rapid method using synthetic nanobodies to inactivate one population. Oppositely oriented Glt proteins showed the same transport kinetics, consistent with the use of an identical gating element on both sides of the membrane. Our work underlines the value of bona fide transport experiments to reveal mechanistic features of Na-aspartate symport that cannot be observed in detergent solution. Combined with previous pre-equilibrium binding studies, a full kinetic mechanism of structurally characterized aspartate transporters of the SLC1A family is now emerging.
External linksCommun Biol / PubMed:34140623 / PubMed Central
MethodsEM (single particle)
Resolution3.5 Å
Structure data

12314

7ngh

EMDB-12314, PDB-7ngh:
Structure of glutamate transporter homologue in complex with Sybody
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-ASP:
ASPARTIC ACID / Aspartic acid

Source
  • thermococcus kodakarensis kod1 (archaea)
  • synthetic construct (others)
KeywordsTRANSPORT PROTEIN / glutamate transporter homologue / GltTk / amino acid transport / membrane protein

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