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TitleStructure of the Yersinia injectisome in intracellular host cell phagosomes revealed by cryo FIB electron tomography.
Journal, issue, pagesJ Struct Biol, Vol. 213, Issue 1, Page 107701, Year 2021
Publish dateFeb 4, 2021
AuthorsCasper Berger / Raimond B G Ravelli / Carmen López-Iglesias / Mikhail Kudryashev / Andreas Diepold / Peter J Peters /
PubMed AbstractMany pathogenic bacteria use the type III secretion system (T3SS), or injectisome, to secrete toxins into host cells. These protruding systems are primary targets for drug and vaccine development. ...Many pathogenic bacteria use the type III secretion system (T3SS), or injectisome, to secrete toxins into host cells. These protruding systems are primary targets for drug and vaccine development. Upon contact between injectisomes and host membranes, toxin secretion is triggered. How this works structurally and functionally is yet unknown. Using cryo-focused ion beam milling and cryo-electron tomography, we visualized injectisomes of Yersinia enterocolitica inside the phagosomes of infected human myeloid cells in a close-to-native state. We observed that a minimum needle length is required for injectisomes to contact the host membrane and bending of host membranes by some injectisomes that contact the host. Through subtomogram averaging, the structure of the entire injectisome was determined, which revealed structural differences in the cytosolic sorting platform compared to other bacteria. These findings contribute to understanding how injectisomes secrete toxins into host cells and provides the indispensable native context. The application of these cryo-electron microscopy techniques paves the way for the study of the 3D structure of infection-relevant protein complexes in host-pathogen interactions.
External linksJ Struct Biol / PubMed:33549695
MethodsEM (subtomogram averaging)
Resolution33.0 - 40.0 Å
Structure data

EMDB-11905:
In situ subtomogram averaging structure of the type III secretion system of yersinia enterocolitica - sorting platform
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

EMDB-11906:
In situ subtomogram averaging structure of the type III secretion system of yersinia enterocolitica - basal body
Method: EM (subtomogram averaging) / Resolution: 33.0 Å

EMDB-11907:
In situ subtomogram averaging structure of the type III secretion system of yersinia enterocolitica - needle tip
Method: EM (subtomogram averaging) / Resolution: 39.0 Å

Source
  • Yersinia enterocolitica (bacteria)

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