EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structure of ssDNA bacteriophage ΦCjT23 provides insight into early virus evolution.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 7478, Year 2022
掲載日	2022年12月3日
著者	Nejc Kejzar / Elina Laanto / Ilona Rissanen / Vahid Abrishami / Muniyandi Selvaraj / Sylvain Moineau / Janne Ravantti / Lotta-Riina Sundberg / Juha T Huiskonen /
PubMed 要旨	The origin of viruses remains an open question. While lack of detectable sequence similarity hampers the analysis of distantly related viruses, structural biology investigations of conserved capsid ...The origin of viruses remains an open question. While lack of detectable sequence similarity hampers the analysis of distantly related viruses, structural biology investigations of conserved capsid protein structures facilitate the study of distant evolutionary relationships. Here we characterize the lipid-containing ssDNA temperate bacteriophage ΦCjT23, which infects Flavobacterium sp. (Bacteroidetes). We report ΦCjT23-like sequences in the genome of strains belonging to several Flavobacterium species. The virion structure determined by cryogenic electron microscopy reveals similarities to members of the viral kingdom Bamfordvirae that currently consists solely of dsDNA viruses with a major capsid protein composed of two upright β-sandwiches. The minimalistic structure of ΦCjT23 suggests that this phage serves as a model for the last common ancestor between ssDNA and dsDNA viruses in the Bamfordvirae. Both ΦCjT23 and the related phage FLiP infect Flavobacterium species found in several environments, suggesting that these types of viruses have a global distribution and a shared evolutionary origin. Detailed comparisons to related, more complex viruses not only expand our knowledge about this group of viruses but also provide a rare glimpse into early virus evolution.
リンク	Nat Commun / PubMed:36463224 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 5.3 Å
構造データ	15042 7zzz EMDB-15042: Icosahedral reconstruction of bacteriophage phiCjT23 capsid PDB-7zzz: Bacteriophage phiCjT23 capsid 手法: EM (単粒子) / 解像度: 4.1 Å 15044 8a01 EMDB-15044: Localized reconstruction of bacteriophage phiCjT23 major capsid protein trimer type 1 PDB-8a01: Bacteriophage phiCjT23 major capsid protein trimer type 1 手法: EM (単粒子) / 解像度: 3.2 Å 15045 8a02 EMDB-15045: Localized reconstruction of bacteriophage phiCjT23 major capsid protein trimer type 2 PDB-8a02: Bacteriophage phiCjT23 major capsid protein trimer type 2 手法: EM (単粒子) / 解像度: 3.2 Å 15046 8a03 EMDB-15046: Localized reconstruction of bacteriophage phiCjT23 major capsid protein trimer type 3 PDB-8a03: Bacteriophage phiCjT23 major capsid protein trimer type 3 手法: EM (単粒子) / 解像度: 3.2 Å 15047 8a04 EMDB-15047: Localized reconstruction of bacteriophage phiCjT23 major capsid protein trimer type 4 PDB-8a04: Bacteriophage phiCjT23 major capsid protein trimer type 4 手法: EM (単粒子) / 解像度: 3.2 Å 15048 8a05 EMDB-15048, PDB-8a05: Bacteriophage phiCjT23 spike protein penton domain 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-15049: Localized reconstruction of bacteriophage phiCjT23 spike 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-15050: Composite map of bacteriophage phiCjT23 capsid calculated from localized reconstructions 手法: EM (単粒子) / 解像度: 3.2 Å 15051 8a06 EMDB-15051: Localized reconstruction of flavobacterium infecting lipid-containing phage FLiP vertex PDB-8a06: Flavobacterium infecting lipid-containing phage FLiP penton protein 手法: EM (単粒子) / 解像度: 4.0 Å
由来	flavobacterium phage (ファージ) unidentified (未定義) flavobacterium phage flip (ファージ)
キーワード	VIRUS (ウイルス) / bacteriophage (ファージ) / phiCjT23 / Flavobacterium (フラボバクテリウム属) / lipid-containing / phage (ファージ) / FLiP / penton protein