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-Structure paper
タイトル | Metallo-supramolecular branched polymer protects particles from air-water interface in single-particle cryo-electron microscopy. |
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ジャーナル・号・ページ | Commun Biol, Vol. 7, Issue 1, Page 65, Year 2024 |
掲載日 | 2024年1月9日 |
著者 | Yixin Xu / Yuqi Qin / Lang Wang / Yingyi Zhang / Yufeng Wang / Shangyu Dang / |
PubMed 要旨 | Recent technological breakthroughs in single-particle cryo-electron microscopy (cryo-EM) enable rapid atomic structure determination of biological macromolecules. A major bottleneck in the current ...Recent technological breakthroughs in single-particle cryo-electron microscopy (cryo-EM) enable rapid atomic structure determination of biological macromolecules. A major bottleneck in the current single particle cryo-EM pipeline is the preparation of good quality frozen cryo-EM grids, which is mostly a trial-and-error process. Among many issues, preferred particle orientation and sample damage by air-water interface (AWI) are common practical problems. Here we report a method of applying metallo-supramolecular branched polymer (MSBP) in the cryo-sample preparation for high-resolution single-particle cryo-EM. Our data shows that MSBP keeps a majority of particles away from air-water interface and mitigates preferred orientation as verified by the analyses of apoferritin, hemagglutinin) trimer and various sample proteins. The use of MSBP is a simple method to improve particle distribution for high-resolution structure determination in single-particle cryo-EM. |
リンク | Commun Biol / PubMed:38195919 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.16 - 3.19 Å |
構造データ | EMDB-36313: Cryo-EM structure of apoferritin with MSBP EMDB-36314: Cryo-EM structure of hemagglutinin with MSBP EMDB-36315: Cryo-EM structure of catalase with MSBP EMDB-36316: Cryo-EM structure of beta-Galactosidase with MSBP |
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