EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for conformational equilibrium of the catalytic spliceosome.
ジャーナル・号・ページ	Mol Cell, Vol. 81, Issue 7, Page 1439-11452.e9, Year 2021
掲載日	2021年4月1日
著者	Max E Wilkinson / Sebastian M Fica / Wojciech P Galej / Kiyoshi Nagai /
PubMed 要旨	The ATPase Prp16 governs equilibrium between the branching (B/C) and exon ligation (C/P) conformations of the spliceosome. Here, we present the electron cryomicroscopy reconstruction of the ...The ATPase Prp16 governs equilibrium between the branching (B/C) and exon ligation (C/P) conformations of the spliceosome. Here, we present the electron cryomicroscopy reconstruction of the Saccharomyces cerevisiae C-complex spliceosome at 2.8 Å resolution and identify a novel C-complex intermediate (C) that elucidates the molecular basis for this equilibrium. The exon-ligation factors Prp18 and Slu7 bind to C before ATP hydrolysis by Prp16 can destabilize the branching conformation. Biochemical assays suggest that these pre-bound factors prime the C complex for conversion to C by Prp16. A complete model of the Prp19 complex (NTC) reveals how the branching factors Yju2 and Isy1 are recruited by the NTC before branching. Prp16 remodels Yju2 binding after branching, allowing Yju2 to remain tethered to the NTC in the C complex to promote exon ligation. Our results explain how Prp16 action modulates the dynamic binding of step-specific factors to alternatively stabilize the C or C conformation and establish equilibrium of the catalytic spliceosome.
リンク	Mol Cell / PubMed:33705709 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 8.0 Å
構造データ	12106 7b9v EMDB-12106: Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound, composite map PDB-7b9v: Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-12107: Yeast C complex spliceosome, focused refinement on core 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-12108: Yeast C complex spliceosome, focused refinement on U2 snRNP 手法: EM (単粒子) / 解像度: 3.58 Å EMDB-12109: Yeast C complex spliceosome, focused refinement on NTC 手法: EM (単粒子) / 解像度: 3.82 Å EMDB-12110: Yeast C complex spliceosome, focused refinement on Brr2/Prp8-Jab1 and Prp16 手法: EM (単粒子) / 解像度: 8.0 Å EMDB-12111: Yeast C complex spliceosome, focused refinement on just Brr2/Prp8-Jab1 手法: EM (単粒子) / 解像度: 7.15 Å EMDB-12112: Yeast C complex spliceosome, overall map after focused classification on Prp17 手法: EM (単粒子) / 解像度: 3.67 Å EMDB-12113: Yeast C complex spliceosome, overall map after focused classification on Slu7 zinc knuckle 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-12114: Yeast C complex spliceosome, overall map after focused classification on Prp18 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-12115: Yeast C complex spliceosome, reconstructed signal-subtracted map after focused classification on Prp19 手法: EM (単粒子) / 解像度: 7.3 Å EMDB-12116: Yeast C complex spliceosome, overall map after focused classification on Prp19 手法: EM (単粒子) / 解像度: 3.97 Å EMDB-12117: Yeast C complex spliceosome, overall map after focused classification on U5 Sm ring 手法: EM (単粒子) / 解像度: 3.13 Å EMDB-12118: Yeast C complex spliceosome, overall map after focused classification on Cwc22 NTD 手法: EM (単粒子) / 解像度: 3.16 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-KGN: D-chiro inositol hexakisphosphate / D-chiro-イノシト-ル六りん酸 ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-ZN*: Unknown entry
由来	saccharomyces cerevisiae (パン酵母) Baker's yeast (パン酵母)
キーワード	SPLICING / spliceosome (スプライセオソーム) / RNA (リボ核酸) / ribozyme (リボザイム)