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-Structure paper
タイトル | Seeing GroEL at 6 A resolution by single particle electron cryomicroscopy. |
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ジャーナル・号・ページ | Structure, Vol. 12, Issue 7, Page 1129-1136, Year 2004 |
掲載日 | 2005年2月9日 |
著者 | Steven J Ludtke / Dong-Hua Chen / Jiu-Li Song / David T Chuang / Wah Chiu / |
PubMed 要旨 | We present a reconstruction of native GroEL by electron cryomicroscopy (cryo-EM) and single particle analysis at 6 A resolution. alpha helices are clearly visible and beta sheet density is also ...We present a reconstruction of native GroEL by electron cryomicroscopy (cryo-EM) and single particle analysis at 6 A resolution. alpha helices are clearly visible and beta sheet density is also visible at this resolution. While the overall conformation of this structure is quite consistent with the published X-ray data, a measurable shift in the positions of three alpha helices in the intermediate domain is observed, not consistent with any of the 7 monomeric structures in the Protein Data Bank model (1OEL). In addition, there is evidence for slight rearrangement or flexibility in parts of the apical domain. The 6 A resolution cryo-EM GroEL structure clearly demonstrates the veracity and expanding scope of cryo-EM and the single particle reconstruction technique for macromolecular machines. |
リンク | Structure / PubMed:15242589 |
手法 | EM (単粒子) |
解像度 | 6.0 Å |
構造データ | EMDB-1081: |
由来 |
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