+
検索条件

キーワード
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

-
Structure paper

タイトル	Emergence of fractal geometries in the evolution of a metabolic enzyme.
ジャーナル・号・ページ	Nature, Vol. 628, Issue 8009, Page 894-900, Year 2024
掲載日	2024年4月10日
著者	Franziska L Sendker / Yat Kei Lo / Thomas Heimerl / Stefan Bohn / Louise J Persson / Christopher-Nils Mais / Wiktoria Sadowska / Nicole Paczia / Eva Nußbaum / María Del Carmen Sánchez Olmos / Karl Forchhammer / Daniel Schindler / Tobias J Erb / Justin L P Benesch / Erik G Marklund / Gert Bange / Jan M Schuller / Georg K A Hochberg /
PubMed 要旨	Fractals are patterns that are self-similar across multiple length-scales. Macroscopic fractals are common in nature; however, so far, molecular assembly into fractals is restricted to synthetic ...Fractals are patterns that are self-similar across multiple length-scales. Macroscopic fractals are common in nature; however, so far, molecular assembly into fractals is restricted to synthetic systems. Here we report the discovery of a natural protein, citrate synthase from the cyanobacterium Synechococcus elongatus, which self-assembles into Sierpiński triangles. Using cryo-electron microscopy, we reveal how the fractal assembles from a hexameric building block. Although different stimuli modulate the formation of fractal complexes and these complexes can regulate the enzymatic activity of citrate synthase in vitro, the fractal may not serve a physiological function in vivo. We use ancestral sequence reconstruction to retrace how the citrate synthase fractal evolved from non-fractal precursors, and the results suggest it may have emerged as a harmless evolutionary accident. Our findings expand the space of possible protein complexes and demonstrate that intricate and regulatable assemblies can evolve in a single substitution.
リンク	Nature / PubMed:38600380 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.71 - 5.91 Å
構造データ	19250 8rjk EMDB-19250, PDB-8rjk: Pseudoatomic model of a second-order Sierpinski triangle formed by the citrate synthase from Synechococcus elongatus 手法: EM (単粒子) / 解像度: 5.91 Å 19251 8rjl EMDB-19251, PDB-8rjl: Structure of a first order Sierpinski triangle formed by the H369R mutant of the citrate synthase from Synechococcus elongatus 手法: EM (単粒子) / 解像度: 3.34 Å PDB-8bp7: Citrate-bound hexamer of Synechococcus elongatus citrate synthase 手法: X-RAY DIFFRACTION / 解像度: 2.71 Å
化合物	ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-CIT: CITRIC ACID / クエン酸 / クエン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER / 水
由来	synechococcus elongatus pcc 7942 = fachb-805 (バクテリア)
キーワード	TRANSFERASE (転移酵素) / Krebs-cycle / TCA-cycle / szierpinski triangle / Fractal complex