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Structure paper

タイトル	Structural basis of exoribonuclease-mediated mRNA transcription termination.
ジャーナル・号・ページ	Nature, Vol. 628, Issue 8009, Page 887-893, Year 2024
掲載日	2024年3月27日
著者	Yuan Zeng / Hong-Wei Zhang / Xiao-Xian Wu / Yu Zhang /
PubMed 要旨	Efficient termination is required for robust gene transcription. Eukaryotic organisms use a conserved exoribonuclease-mediated mechanism to terminate the mRNA transcription by RNA polymerase II ...Efficient termination is required for robust gene transcription. Eukaryotic organisms use a conserved exoribonuclease-mediated mechanism to terminate the mRNA transcription by RNA polymerase II (Pol II). Here we report two cryogenic electron microscopy structures of Saccharomyces cerevisiae Pol II pre-termination transcription complexes bound to the 5'-to-3' exoribonuclease Rat1 and its partner Rai1. Our structures show that Rat1 displaces the elongation factor Spt5 to dock at the Pol II stalk domain. Rat1 shields the RNA exit channel of Pol II, guides the nascent RNA towards its active centre and stacks three nucleotides at the 5' terminus of the nascent RNA. The structures further show that Rat1 rotates towards Pol II as it shortens RNA. Our results provide the structural mechanism for the Rat1-mediated termination of mRNA transcription by Pol II in yeast and the exoribonuclease-mediated termination of mRNA transcription in other eukaryotes.
リンク	Nature / PubMed:38538796
手法	EM (単粒子)
解像度	2.7 - 2.8 Å
構造データ	36162 8jch EMDB-36162, PDB-8jch: Cryo-EM structure of yeast Rat1-bound Pol II pre-termination transcription complex 1 (Pol II Rat1-PTTC1) 手法: EM (単粒子) / 解像度: 2.7 Å 36908 8k5p EMDB-36908, PDB-8k5p: Cryo-EM structure of yeast Rat1-bound Pol II pre-termination transcription complex 2 (Pol II Rat1-PTTC2) 手法: EM (単粒子) / 解像度: 2.8 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae s288c (パン酵母) synthetic construct (人工物)
キーワード	TRANSCRIPTION (転写 (生物学)) / Transcription termination / Pol II / Rat1/Rai1 / Spt5 / Rat1/Rai1 Spt5