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Structure paper

TitleMolecular Basis for Extender Unit Specificity of Mycobacterial Polyketide Synthases.
Journal, issue, pagesAcs Chem. Biol., Vol. 15, Page 3206-3216, Year 2020
Publish dateSep 23, 2020 (structure data deposition date)
AuthorsGrabowska, A.D. / Brison, Y. / Maveyraud, L. / Gavalda, S. / Faille, A. / Nahoum, V. / Bon, C. / Guilhot, C. / Pedelacq, J.D. / Chalut, C. / Mourey, L.
External linksAcs Chem. Biol. / PubMed:33237724
MethodsX-ray diffraction
Resolution1.6 - 3.1 Å
Structure data

PDB-7agp:
Structure of the AcylTransferase domain of Mycocerosic Acid Synthase from Mycobacterium tuberculosis
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-7agq:
Structure of the S726F mutant of AcylTransferase domain of Mycocerosic Acid Synthase from Mycobacterium tuberculosis
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-7agr:
Structure of the M624V-S726F mutant of AcylTransferase domain of Mycocerosic Acid Synthase from Mycobacterium tuberculosis soaked with MethylMalonyl Coenzyme A
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-7ags:
Structure of the AcylTransferase domain of Mycocerosic Acid Synthase from Mycobacterium tuberculosis acylated MethylMalonyl-CoenzymeA
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-7agt:
Structure of the S726F mutant of AcylTransferase domain of Mycocerosic Acid Synthase from Mycobacterium tuberculosis acylated with Malonyl-coenzyme A
Method: X-RAY DIFFRACTION / Resolution: 2.901 Å

PDB-7agu:
Structure of the S726F mutant of AcylTransferase domain of Mycocerosic Acid Synthase from Mycobacterium tuberculosis acylated with MethylMalonyl-coenzyme A
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-7ahb:
Acyltransferase domain of the polyketide synthase PpsC of Mycobacterium tuberculosis
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7akc:
Structure of the of AcylTransferase domain of phenolphthiocerol/phtiocerol synthase A from Mycobacterium bovis (BCG)
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL / Ethylene glycol

ChemComp-HOH:
WATER / Water

ChemComp-ACT:
ACETATE ION / Acetate

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-DXX:
METHYLMALONIC ACID / Methylmalonic acid

ChemComp-MLA:
MALONIC ACID / Malonic acid

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-SCN:
THIOCYANATE ION / Thiocyanate

ChemComp-NA:
Unknown entry

Source
  • mycobacterium bovis (strain atcc baa-935 / af2122/97) (bacteria)
  • mycobacterium tuberculosis (strain atcc 25618 / h37rv) (bacteria)
  • mycobacterium bovis (strain bcg / pasteur 1173p2) (bacteria)
KeywordsTRANSFERASE / Mycocerosic Acid Synthase / AcylTransferase domain / polyketide synthase / Mycobacterium tuberculosis / methylmalonyl-Coenzyme A / malonyl-coenzyme A / PpsC / acyltransferase / Mycobacterium turberculosis / phenolphtiocerol/phtiocerol synthase A; acyl transferase; Polyketide synthase; Mycobacterium bovis

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