EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism.
ジャーナル・号・ページ	Nat Commun, Vol. 10, Issue 1, Page 2754, Year 2019
掲載日	2019年6月21日
著者	Valentin Mitterer / Ramtin Shayan / Sébastien Ferreira-Cerca / Guillaume Murat / Tanja Enne / Dana Rinaldi / Sarah Weigl / Hajrija Omanic / Pierre-Emmanuel Gleizes / Dieter Kressler / Celia Plisson-Chastang / Brigitte Pertschy /
PubMed 要旨	Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is ...Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is poorly understood. Using functional analyses and cryo-EM, we show that ribosomal protein Rps20 orchestrates communication between two multi-step maturation events across the pre-40S subunit. Our study reveals that during pre-40S maturation, formation of essential contacts between Rps20 and Rps3 permits assembly factor Ltv1 to recruit the Hrr25 kinase, thereby promoting Ltv1 phosphorylation. In parallel, a deeply buried Rps20 loop reaches to the opposite pre-40S side, where it stimulates Rio2 ATPase activity. Both cascades converge to the final maturation steps releasing Rio2 and phosphorylated Ltv1. We propose that conformational proofreading exerted via Rps20 constitutes a checkpoint permitting assembly factor release and progression of pre-40S maturation only after completion of all earlier maturation steps.
リンク	Nat Commun / PubMed:31227701 / PubMed Central
手法	EM (単粒子)
解像度	3.15 - 3.8 Å
構造データ	4792 6rbd 6rbe EMDB-4792, PDB-6rbd: State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles PDB-6rbe: State 2 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles 手法: EM (単粒子) / 解像度: 3.47 Å 4793 6rbd 6rbe EMDB-4793, PDB-6rbe: State 2 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles PDB-6rbd: State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles 手法: EM (単粒子) / 解像度: 3.8 Å 4794 6rbd 6rbe EMDB-4794: Yeast pre-40S Rps20 DeltaLoop C1-Headonly PDB-6rbd: State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles PDB-6rbe: State 2 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles 手法: EM (単粒子) / 解像度: 3.8 Å 4795 6rbd 6rbe EMDB-4795: Yeast pre-40S Rps20 DeltaLoop C2-Headonly PDB-6rbd: State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles PDB-6rbe: State 2 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles 手法: EM (単粒子) / 解像度: 3.8 Å 4796 6rbd 6rbe EMDB-4796: yeast pre-40S particle - focus on Dim1 PDB-6rbd: State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles PDB-6rbe: State 2 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles 手法: EM (単粒子) / 解像度: 3.15 Å
由来	saccharomyces cerevisiae s288c (パン酵母) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母)
キーワード	RIBOSOME (リボソーム) / pre-40S / yeast (酵母) / cytoplasmic (細胞質) / mutant Rps20