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TitleUbp6 deubiquitinase controls conformational dynamics and substrate degradation of the 26S proteasome.
Journal, issue, pagesNat Struct Mol Biol, Vol. 22, Issue 9, Page 712-719, Year 2015
Publish dateAug 24, 2015
AuthorsCharlene Bashore / Corey M Dambacher / Ellen A Goodall / Mary E Matyskiela / Gabriel C Lander / Andreas Martin /
PubMed AbstractSubstrates are targeted for proteasomal degradation through the attachment of ubiquitin chains that need to be removed by proteasomal deubiquitinases before substrate processing. In budding yeast, ...Substrates are targeted for proteasomal degradation through the attachment of ubiquitin chains that need to be removed by proteasomal deubiquitinases before substrate processing. In budding yeast, the deubiquitinase Ubp6 trims ubiquitin chains and affects substrate processing by the proteasome, but the underlying mechanisms and the location of Ubp6 within the holoenzyme have been elusive. Here we show that Ubp6 activity strongly responds to interactions with the base ATPase and the conformational state of the proteasome. Electron microscopy analyses reveal that ubiquitin-bound Ubp6 contacts the N ring and AAA+ ring of the ATPase hexamer and is in proximity to the deubiquitinase Rpn11. Ubiquitin-bound Ubp6 inhibits substrate deubiquitination by Rpn11, stabilizes the substrate-engaged conformation of the proteasome and allosterically interferes with the engagement of a subsequent substrate. Ubp6 may thus act as a ubiquitin-dependent 'timer' to coordinate individual processing steps at the proteasome and modulate substrate degradation.
External linksNat Struct Mol Biol / PubMed:26301997 / PubMed Central
MethodsEM (single particle)
Resolution22.3 - 25.2 Å
Structure data

EMDB-2995:
Negative stain 3D reconstruction of the yeast 26S proteasome in complex with ubiquitin-bound Ubp6
Method: EM (single particle) / Resolution: 22.3 Å

EMDB-6334:
Negative stain 3D reconstruction of the yeast 26S proteasome in ATPgS in the presence of wild-type Ubp6 protein
Method: EM (single particle) / Resolution: 25.2 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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