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TitleLis1 regulates dynein by sterically blocking its mechanochemical cycle.
Journal, issue, pagesElife, Vol. 3, Year 2014
Publish dateNov 7, 2014
AuthorsKaterina Toropova / Sirui Zou / Anthony J Roberts / William B Redwine / Brian S Goodman / Samara L Reck-Peterson / Andres E Leschziner /
PubMed AbstractRegulation of cytoplasmic dynein's motor activity is essential for diverse eukaryotic functions, including cell division, intracellular transport, and brain development. The dynein regulator Lis1 is ...Regulation of cytoplasmic dynein's motor activity is essential for diverse eukaryotic functions, including cell division, intracellular transport, and brain development. The dynein regulator Lis1 is known to keep dynein bound to microtubules; however, how this is accomplished mechanistically remains unknown. We have used three-dimensional electron microscopy, single-molecule imaging, biochemistry, and in vivo assays to help establish this mechanism. The three-dimensional structure of the dynein-Lis1 complex shows that binding of Lis1 to dynein's AAA+ ring sterically prevents dynein's main mechanical element, the 'linker', from completing its normal conformational cycle. Single-molecule experiments show that eliminating this block by shortening the linker to a point where it can physically bypass Lis1 renders single dynein motors insensitive to regulation by Lis1. Our data reveal that Lis1 keeps dynein in a persistent microtubule-bound state by directly blocking the progression of its mechanochemical cycle.
External linksElife / PubMed:25380312 / PubMed Central
MethodsEM (single particle)
Resolution14.8 - 23.1 Å
Structure data

EMDB-6008:
Dynein motor domain in complex with Lis1 in the absence of nucleotide
Method: EM (single particle) / Resolution: 21.4 Å

EMDB-6013:
Dynein motor domain in the absence of nucleotide
Method: EM (single particle) / Resolution: 14.8 Å

EMDB-6014:
Dynein motor domain in the presence of ADP, with linker at position 2
Method: EM (single particle) / Resolution: 19.5 Å

EMDB-6015:
Dynein motor domain in the presence of ADP, with linker at position 1
Method: EM (single particle) / Resolution: 18.3 Å

EMDB-6016:
Dynein motor domain in complex with Lis1 in the presence of ATP and Vi
Method: EM (single particle) / Resolution: 23.1 Å

EMDB-6017:
Dynein motor domain with truncated linker in complex with Lis1 in the absence of nucleotide
Method: EM (single particle) / Resolution: 15.4 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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