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Title | Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by electron cryo-microscopy. |
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Journal, issue, pages | J Gen Virol, Vol. 92, Issue Pt 8, Page 1960-1970, Year 2011 |
Publish date | May 11, 2011 |
Authors | Jennifer L Miller / Jeremy Woodward / Shaoxia Chen / Mohammed Jaffer / Brandon Weber / Keizo Nagasaki / Yuji Tomaru / Roger Wepf / Alan Roseman / Arvind Varsani / Trevor Sewell / |
PubMed Abstract | Heterocapsa circularisquama RNA virus is a non-enveloped icosahedral ssRNA virus infectious to the harmful bloom-forming dinoflagellate, H. circularisquama, and which is assumed to be the major ...Heterocapsa circularisquama RNA virus is a non-enveloped icosahedral ssRNA virus infectious to the harmful bloom-forming dinoflagellate, H. circularisquama, and which is assumed to be the major natural agent controlling the host population. The viral capsid is constructed from a single gene product. Electron cryo-microscopy revealed that the virus has a diameter of 34 nm and T = 3 symmetry. The 180 quasi-equivalent monomers have an unusual arrangement in that each monomer contributes to a 'bump' on the surface of the protein. Though the capsid protein probably has the classic 'jelly roll' β-sandwich fold, this is a new packing arrangement and is distantly related to the other positive-sense ssRNA virus capsid proteins. The handedness of the structure has been determined by a novel method involving high resolution scanning electron microscopy of the negatively stained viruses and secondary electron detection. |
External links | J Gen Virol / PubMed:21562120 |
Methods | EM (single particle) |
Resolution | 18.0 Å |
Structure data | EMDB-1870: |
Source |
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