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TitleThree-dimensional reconstruction of Heterocapsa circularisquama RNA virus by electron cryo-microscopy.
Journal, issue, pagesJ Gen Virol, Vol. 92, Issue Pt 8, Page 1960-1970, Year 2011
Publish dateMay 11, 2011
AuthorsJennifer L Miller / Jeremy Woodward / Shaoxia Chen / Mohammed Jaffer / Brandon Weber / Keizo Nagasaki / Yuji Tomaru / Roger Wepf / Alan Roseman / Arvind Varsani / Trevor Sewell /
PubMed AbstractHeterocapsa circularisquama RNA virus is a non-enveloped icosahedral ssRNA virus infectious to the harmful bloom-forming dinoflagellate, H. circularisquama, and which is assumed to be the major ...Heterocapsa circularisquama RNA virus is a non-enveloped icosahedral ssRNA virus infectious to the harmful bloom-forming dinoflagellate, H. circularisquama, and which is assumed to be the major natural agent controlling the host population. The viral capsid is constructed from a single gene product. Electron cryo-microscopy revealed that the virus has a diameter of 34 nm and T = 3 symmetry. The 180 quasi-equivalent monomers have an unusual arrangement in that each monomer contributes to a 'bump' on the surface of the protein. Though the capsid protein probably has the classic 'jelly roll' β-sandwich fold, this is a new packing arrangement and is distantly related to the other positive-sense ssRNA virus capsid proteins. The handedness of the structure has been determined by a novel method involving high resolution scanning electron microscopy of the negatively stained viruses and secondary electron detection.
External linksJ Gen Virol / PubMed:21562120
MethodsEM (single particle)
Resolution18.0 Å
Structure data

EMDB-1870:
Three-Dimensional Reconstruction of Heterocapsa circularisquama RNA Virus (HcRNAV-109) by Cryo-Electron Microscopy
Method: EM (single particle) / Resolution: 18.0 Å

Source
  • Heterocapsa circularisquama (eukaryote)

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