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TitleStructural convergence between Cryo-EM and NMR reveals intersubunit interactions critical for HIV-1 capsid function.
Journal, issue, pagesCell, Vol. 139, Issue 4, Page 780-790, Year 2009
Publish dateNov 13, 2009
AuthorsIn-Ja L Byeon / Xin Meng / Jinwon Jung / Gongpu Zhao / Ruifeng Yang / Jinwoo Ahn / Jiong Shi / Jason Concel / Christopher Aiken / Peijun Zhang / Angela M Gronenborn /
PubMed AbstractMature HIV-1 particles contain conical-shaped capsids that enclose the viral RNA genome and perform essential functions in the virus life cycle. Previous structural analysis of two- and three- ...Mature HIV-1 particles contain conical-shaped capsids that enclose the viral RNA genome and perform essential functions in the virus life cycle. Previous structural analysis of two- and three-dimensional arrays of the capsid protein (CA) hexamer revealed three interfaces. Here, we present a cryoEM study of a tubular assembly of CA and a high-resolution NMR structure of the CA C-terminal domain (CTD) dimer. In the solution dimer structure, the monomers exhibit different relative orientations compared to previous X-ray structures. The solution structure fits well into the EM density map, suggesting that the dimer interface is retained in the assembled CA. We also identified a CTD-CTD interface at the local three-fold axis in the cryoEM map and confirmed its functional importance by mutagenesis. In the tubular assembly, CA intermolecular interfaces vary slightly, accommodating the asymmetry present in tubes. This provides the necessary plasticity to allow for controlled virus capsid dis/assembly.
External linksCell / PubMed:19914170 / PubMed Central
MethodsEM (helical sym.) / NMR (solution)
Resolution16.0 Å
Structure data

EMDB-5136:
CryoEM Structure of Tubular Assembly of HIV-1 CA and Evidence for a Novel Interface
Method: EM (helical sym.) / Resolution: 16.0 Å

PDB-2kod:
A high-resolution NMR structure of the dimeric C-terminal domain of HIV-1 CA
Method: SOLUTION NMR

Source
  • Human immunodeficiency virus
  • hiv-1 m:b_hxb2r (virus)
KeywordsVIRAL PROTEIN / HIV-1 capsid / C-terminal domain

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