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| Title | RNA genome packaging and capsid assembly of bluetongue virus visualized in host cells. |
|---|---|
| Journal, issue, pages | Cell, Vol. 187, Issue 9, Page 2236-2249.e17, Year 2024 |
| Publish date | Apr 25, 2024 |
 Authors | Xian Xia / Po-Yu Sung / Michael W Martynowycz / Tamir Gonen / Polly Roy / Z Hong Zhou /   |
| PubMed Abstract | Unlike those of double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and ssRNA viruses, the mechanism of genome packaging of dsRNA viruses is poorly understood. Here, we combined the techniques ...Unlike those of double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and ssRNA viruses, the mechanism of genome packaging of dsRNA viruses is poorly understood. Here, we combined the techniques of high-resolution cryoelectron microscopy (cryo-EM), cellular cryoelectron tomography (cryo-ET), and structure-guided mutagenesis to investigate genome packaging and capsid assembly of bluetongue virus (BTV), a member of the Reoviridae family of dsRNA viruses. A total of eleven assembly states of BTV capsid were captured, with resolutions up to 2.8 Å, with most visualized in the host cytoplasm. ATPase VP6 was found underneath the vertices of capsid shell protein VP3 as an RNA-harboring pentamer, facilitating RNA packaging. RNA packaging expands the VP3 shell, which then engages middle- and outer-layer proteins to generate infectious virions. These revealed "duality" characteristics of the BTV assembly mechanism reconcile previous contradictory co-assembly and core-filling models and provide insights into the mysterious RNA packaging and capsid assembly of Reoviridae members and beyond. |
 External links | Cell / PubMed:38614100 |
| Methods | EM (single particle) / EM (subtomogram averaging) |
| Resolution | 2.8 - 7.2 Å |
| Structure data | EMDB-43714, PDB-8w12: EMDB-43716, PDB-8w19: EMDB-43719, PDB-8w1c:  EMDB-43722: Cryo-EM structure of pre-subcore from in vitro assembled particles  EMDB-43723: Cryo-EM structure of BTV empty virion  EMDB-43724: Cryo-EM structure of BTV empty core  EMDB-43725: Cryo-EM structure of BTV empty pre-core EMDB-43726, PDB-8w1i: EMDB-43727, PDB-8w1o:  EMDB-43728: Subtomogram averaging of BTV virion in host cells EMDB-43730, PDB-8w1r: EMDB-43731, PDB-8w1s: |
| Source |
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 Keywords |  VIRAL PROTEIN / Bluetongue virus / capsid protein / heterohexamer / assembly intermediate / pre-subcore / virion / core |
