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| Title | Structure of the γ-tubulin ring complex-capped microtubule. |
|---|---|
| Journal, issue, pages | Nat Struct Mol Biol, Year 2024 |
| Publish date | Apr 12, 2024 |
 Authors | Amol Aher / Linas Urnavicius / Allen Xue / Kasahun Neselu / Tarun M Kapoor /  |
| PubMed Abstract | Microtubules are composed of α-tubulin and β-tubulin dimers positioned head-to-tail to form protofilaments that associate laterally in varying numbers. It is not known how cellular microtubules ...Microtubules are composed of α-tubulin and β-tubulin dimers positioned head-to-tail to form protofilaments that associate laterally in varying numbers. It is not known how cellular microtubules assemble with the canonical 13-protofilament architecture, resulting in micrometer-scale α/β-tubulin tracks for intracellular transport that align with, rather than spiral along, the long axis of the filament. We report that the human ~2.3 MDa γ-tubulin ring complex (γ-TuRC), an essential regulator of microtubule formation that contains 14 γ-tubulins, selectively nucleates 13-protofilament microtubules. Cryogenic electron microscopy reconstructions of γ-TuRC-capped microtubule minus ends reveal the extensive intra-domain and inter-domain motions of γ-TuRC subunits that accommodate luminal bridge components and establish lateral and longitudinal interactions between γ-tubulins and α-tubulins. Our structures suggest that γ-TuRC, an inefficient nucleation template owing to its splayed conformation, can transform into a compacted cap at the microtubule minus end and set the lattice architecture of cellular microtubules. |
 External links | Nat Struct Mol Biol / PubMed:38609661 |
| Methods | EM (single particle) |
| Resolution | 2.66 - 8.5 Å |
| Structure data | EMDB-43482: Density map for gamma tubulin ring complex capped microtubule EMDB-43483: Refined density map of gamma tubulin ring complex capped microtubule EMDB-43519, PDB-8vt7: |
| Chemicals |  ChemComp-GDP:  ChemComp-GTP:  ChemComp-MG: |
| Source |
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 Keywords |  CELL CYCLE / Microtubule nucleation complex bound to a microtubule / Microtubule |
